Search academic texts

Information × Registration Number 0224U033333, (0123U100780) , R & D reports Title Development of modern methods of marker-assisted selection and technologies of short interfering RNAs for the creation of high-yielding varieties-innovations of winter wheat with improved grain quality, resistant to environmental stresses. popup.stage_title Комплексне вивчення простих, складних та бекросних гібридів різних поколінь генетико-фізіологічними методами. Польова та лабораторна оцінка генетичного матеріалу за допомогою молекулярних маркерних систем. Отримання методом in planta та аналіз трансгенних рослин пшениці з дволанцюговим РНК-супресором гена проліндегідрогенази. Аналіз отриманих генотипів за ознаками продуктивності, стійкості до несприятливих факторів довкілля та якості зерна. Виконання комплексу робіт по первинному насінництву та впровадженню рослинницької продукції у виробництво. Head Morhun Volodymyr V., Доктор біологічних наук Registration Date 25-12-2024 Organization Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine popup.description1 The purpose of the work is to develop modern methods of marker-assisted selection and technologies of short interfering RNA for their use in the selection process to increase the potential productivity and improve the quality of winter soft wheat grain, as well as the adaptive potential of plants to adverse environmental conditions and obtain on this basis new varieties-innovations. popup.description2  Marker systems were selected to determine the allelic composition of high-molecular glutenin loci in soft winter wheat lines for the selection of genotypes with high baking quality of flour. A system of DNA markers was developed to identify centric wheat-rye translocations 1AL.1RS and 1ВL.1RS. Based on our own practical developments, individual elements of the technology for creating transgenic winter wheat plants by the Agrobacterium-mediated transformation in planta method were improved, which contributes to obtaining a higher percentage of genetically modified variants and accelerates the process of obtaining modified plants with increased tolerance to stresses associated with water deficiency. It was shown that the increased level of proline in transformants contributed to an increase in the drought resistance of the photosynthetic apparatus according to the parameters of the intensity of CO2 assimilation, transpiration, stomatal conductivity and efficiency of water use during photosynthesis. It was established that the extension of the duration of the flag leaf functioning during the reproductive period and the higher activity of the photosynthetic apparatus in transgenic wheat plants with an increased proline content under stressful conditions caused an increase in their grain productivity. Valuable wheat breeding material with unique tolerance to drought in the germination phase was isolated, as well as genetic material tolerant to critical conditions of provocative moistening. It was established that the highest tolerance to critical drought in the germination phase and the highest viability was shown by breeding material with black grain, which combines blue and purple grain colors in one genotype. The only and numerous collection of source material with purple, blue and black grain was collected in Ukraine. To enhance the antioxidant activity of grain, genotypes with a high content of carotenoids in grain and yellow flour color were involved in the crossi Product Description popup.authors Velykozhon Liudmyla H. Dubrovna Oksana V. Morhun Bohdan V. Priadkina Halyna O. Radchenko Оleksii M. Rybalka Oleksandr I. Sandetska Nadiia V. Skryplov Volodymyr O. Yakymchuk Ruslan A. popup.nrat_date 2024-12-25 Close