Search academic texts

Information × Registration Number 0224U033405, (0123U103026) , R & D reports Title Identification of ivermectin binding site on tubulin of phytonematodes and investigation of its nematicide activity popup.stage_title Дослідження нематоцидного впливу івермектину на фітонематоди та захисного - на потенційні рослини, які вони здатні заражати Head Buziashvili Anastasiia Yu., Кандидат біологічних наук Registration Date 26-12-2024 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description1 Investigation of the influence of ivermectin on different species of phytopathogenic nematodes and plants which they infect on molecular, cellular and organismic levels. Determination of the nematicide activity of ivermectin in silico and in vitro on the most common species of phytopathogenic nematodes infecting potato and sugar beet. Evaluation of the effects of ivermectin on sugar beet and potato plants and identification of its phytoprotective properties after inoculation in vitro with nematodes. popup.description2  It was established that the aminoacid sequences of β-tubulin of the nematodes (Globodera pallida, Melodogyne enterolobii and M. graminicola,) and plants (Beta vulgaris, Solanum tuberosum) are highly conserved. Among the elements of secondary structure, the most conserved were H1, H6 and H7 spirals, and the least conserved – disordered regions H6-H7, М-loop and S9-S10. Also, the interaction between IVM and β-tubulin of the studied organisms was first modelled and the stability of all complexes was established using molecular dynamics. The highest affinity of IVM was to β-tubulin of M. enterolobii (ΔGbind=-8,49±3,92 ккал/моль), and the lowest – to β-tubulin of S. tuberosum ( ΔGbind=-3,77±3,93 ккал/моль). The affinity of IVM to β-tubulin of phytoparasitic nematodes was higher than to β-tubulin of plants, which emphasizes its potential as a plant protection agent. Also, the influence of IVM on morpho-physiological parameters of plants depended on organic solvent. The shoot length and total leaf area of potato plants in vitro in the presence of 10 μg/ml IVM were 57,3% and 170,79% higher than the control when the solution of IVM in methanol was used. The fresh weight and root length of the sugar beet seedlings in the presence of 10 μg/ml IVM were 48,94% and 29,09% higher than the control. Moreover, the potato plants grown in vivo in the soil infected with Globodera rostochiensis and Ditylenchus destructor nematodes and watered twice with the IVM solutions had 1,51 times higher shoots, 3,56 times larger total leaf area and 4,05 times heavier tubers than the control ones. The average number of cysts, eggs in cysts and the total number of eggs and juveniles of G. rostoсhiensis in the soil samples in which the potato plants treated with IVM were grown, were 2,4, 2,73 and 6,55 times lower, than in control. The frequency of potato tubers damage by D. destructor in the control was 30,76%, and in the samples treated with 10, 50 and 100 μg/ml IVM were 0%, 23,07% and 9,09%. Product Description popup.authors Buziashvili Anastasiia Yu. Kustovskyi Yevhen O. popup.nrat_date 2024-12-26 Close