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Information × Registration Number 0224U033434, (0120U100381) , R & D reports Title Cryopreservation of spermatogonial stem and accessory regulatory cells of the testes with using of biopolymer gels. popup.stage_title Визначення композиційного складу кріозахисного середовища для кріоконсервування сперматогоніальних стовбурових та акцесорно-регуляторних клітин сім’яників з використанням біополімерних гелів. Head Holtsev Anatolii M., Доктор медичних наук Registration Date 27-12-2024 Organization Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine popup.description1 Experimental reasoning of the possibility of using biopolymer gels to improve the preservation of the morphofunctional state of spermatogonial stem and accessory-regulatory cells of the testes of rat at the stages of cryopreservation popup.description2  Biopolymer gels attract a lot of attention in a field of biothechnology due to their excellent compatibility and degradation. Their application is also promising for cryopreservtion of spermatogonial stem cells (SSCs) which is so necessary to preserve the fertility of young patients. The aim of the study was to determine the effectiveness of biopolymer gels as a component of cryopreservation medium for SSCs of immature rats at the stage of exposure to cryoprotectants. It was found that 30-min exposure to cryopreservation media based on collagen or fibrin gel with an addition of 5% Me2SO or 6% glycerol did not lead to significant changes in membrane integrity, cytochrome C content, metabolic, mitochondrial and antioxidant activities in SSCs compared to the control (Leibovitz-based cryomedium). But fibrin gel more than collagen reduced the toxic effects of Me2SO and glycerol on SSCs increasing exposure time up to 45 min without significant changes in cell viability. The same cryoprotectants in Leibovitz-based media showed significant toxicity starting from the 15th minute of exposure. Necrosis was the main cause of cell death at this stage of cryopreservation in all experimental groups. The obtained results can be used to optimize SSC cryopreservation protocols for further cell autotransplantation for spermatogenesis initiation in boys who undergo gonadotoxic therapy in prepubertal age. Product Description popup.authors Volkova Nataliia O. Sokil Larysa V. Stepaniuk Liudmyla V. Chernyshenko Liudmyla H. Yukhta Mariia S. popup.nrat_date 2024-12-27 Close