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Information × Registration Number 0224U033522, (0123U100040) , R & D reports Title Study of the anti-burn effect, properties of biopolymer materials with immobilized serratiopeptidase and the effect of metal ions on the activity of the native enzyme using computer technologies popup.stage_title Звіт з наукової роботи, наукові публікації. Дослідження збереження біохімічних властивостей розроблених стерильних біополімерних матеріалів: вміст білка, комплексної протеолітичної активності і фізико-хімічних характеристик (рН- і термозалежність, рН- і термостабільність активності) протягом тривалого часу в умовах низьких температур, порівняння біохімічних властивостей розроблених стерильних біополімерних матеріалів з референс препаратом протиопікової дії «Іруксол» та вивчення впливу іонів металів на каталітичну активність серратіопептидази із застосуванням методів комп’ютерної хімії (QSAR-аналіз). Head Dekina Svitlana S., Доктор біологічних наук Registration Date 29-12-2024 Organization Physico-Chemical Institute O. Bogatsky National Academy of Sciences of Ukraine popup.description1 The purpose of the work is to study the in vivo anti-burn effect of the developed sterile biopolymer materials of complex proteolytic and antimicrobial activity with immobilized serratiopeptidase enzyme: gauze bandage and gel, study of their biochemical and physicochemical properties (protein content, proteolytic activity, pH and temperature dependence, pH - and thermostability of activity) during long-term storage, study of the influence of metal ions on the catalytic activity of serratiopeptidase by the method of QSAR analysis. popup.description2  Research objects: Na-CMC gel with incorporated serratiopeptidase Serratia E15 and two-layer gauze dressings with immobilized serratiopeptidase using PVA/sodium alginate. The purpose of the study is to investigate the biochemical and physicochemical characteristics of the developed sterile biopolymer materials (Na-CMC gel with incorporated serratiopeptidase and gauze dressings with immobilized serratiopeptidase using PVA/sodium alginate) and to compare them with those of the reference burn treatment preparation Iruxol. Additionally, the study aims to analyze the effect of metal ions on the catalytic activity of the enzyme serratiopeptidase using computational chemistry methods. For the study, experimental batches of Na-CMC gel with incorporated serratiopeptidase and two-layer gauze dressings with serratiopeptidase using PVA/sodium alginate and the addition of the antiseptic chlorhexidine bigluconate were prepared. The resulting bioproducts maintained a high level of activity (over 92%) for two years when stored at 0–4 °C. The physicochemical characteristics of the developed immobilized serratiopeptidase preparations were compared with those of collagenase from Clostridium histolyticum in the reference preparation Iruxol. It was demonstrated that the thermal profiles and temperature stability of the enzymes are quite similar. However, under simulated wound environment conditions (pH 5.5), serratiopeptidase exhibited 6.4–7.5 times higher activity compared to collagenase. The effect of metal ions on the retention of serratiopeptidase proteolytic activity was studied. Some metal ions (Li+, Mn2+, and Co2+) activated the peptidase, while others (Cu2+, Be2+, Cd2+, Hg2+, Cr3+) inhibited enzyme activity. Using multiple linear regression, a statistically adequate model with high predictive capability was developed under cross-validation and for the test dataset. Product Description popup.authors Dekina Svitlana S. Toptikov Valentyn A. Shesterenko Yevheniia А. Shesterenko Yuliia A. popup.nrat_date 2024-12-29 Close