Search academic texts

Information × Registration Number 0224U033561, (0123U104137) , R & D reports Title Development of a gel with wound-healing properties for external use popup.stage_title Оптимізація умов одержання та очистки рекомбінантного аналогу гепарин-зв’язувального епідермального фактору росту людини (rhHB-EGF). Розроблення лабораторного технологічного регламенту одержання rhHB-EGF. Біологічна стандартизація та оцінка регенеративного потенціалу субстанції rhHB-EGF. Розроблення складу та технології одержання гелю для зовнішнього застосування. Аналітична стандартизація та оцінка безпечності розробленого засобу. Біологічна оцінка безпечності засобу методами in vivo. Розроблення рекомендацій щодо медичного застосування на основі клінічної оцінки засобу. Розроблення технічних умов на гель для зовнішнього застосування. Head Kolybo Denys V., Доктор біологічних наук Registration Date 30-12-2024 Organization Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine popup.description1 The aim of the project is to develop the composition and manufacturing technology of a gel based on recombinant heparin-binding human epidermal growth factor (rhHB-EGF) and to investigate its ability to influence regenerative processes during wound healing. popup.description2 The conditions for obtaining and purifying the recombinant analog of human heparin-binding EGF-like growth factor (rhHB-EGF) were optimized. The laboratory procedure for obtaining a functionally active form of the rhHB-EGF using E. coli producer strains has been developed. The biological activity of the obtained rhHB-EGF was confirmed in cell culture. The composition and manufacturing technology of the drug in gel form with wound-healing properties for external use containing rhHB-EGF have been developed. Analytical standardization of the developed gel was carried out (the optimal ratio of gel components was determined, their stability was investigated, and analytical methods were developed to determine its components). It was shown that the drug in gel form for external use containing rhHB-EGF did not cause complications in the model of excisional skin wounds in mice and had the maximal effect on the morphometric parameters of skin wound regeneration on the fifth day after application. The safety of the developed gel has been confirmed using in vitro and in vivo methods. The project of technical conditions for the gel with wound-healing properties has been created, and recommendations for the use of this product have also been proposed. The development of new, more effective drugs for wound healing is an important task because of the large number of wounded during the war and the presence of a significant number of patients with chronic wounds that are difficult to treat (due to diabetes, bedsores, etc.).  Product Description popup.authors Vovk Iryna O. Halkin Oleksandr Yu. Holembiovska Оlena I. Horbаtiuk Oksаnа B. Dovbynchuk Taisa V. Dronko Liliia M. Zukova Dariia A. Kyryk Vitalii M. Komisarenko Serhii V. Lutsenko Tetiana M. Romaniuk Svitlana I. Siromolot Andrii A. Usenko Mariia O. popup.nrat_date 2024-12-30 Close