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Information × Registration Number 0224U033562, (0121U108920) , R & D reports Title To study the population-genetic variability of valuable fish farming objects in the process of domestication popup.stage_title Здійснити оцінку популяційної і видової мінливості для визначення структури стада, генетичного різноманіття різновікових груп культивованих риб Head Mariutsa Alla E., Кандидат сільськогосподарських наук Registration Date 30-12-2024 Organization Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine popup.description1 The project aims to develop scientific principles of population-genetic control of valuable aquaculture objects (sturgeon, salmon fish, etc.) and to determine methods of effective use of the gene pool of cultivated fish species in conditions of domestication using molecular genetic and cytogenetic methods popup.description2  The micronucleus test revealed that the studied group of Siberian sturgeon exhibited low levels of erythrocytes with micronuclei (EMN = 3.6 ± 0.3‰). Additionally, the group demonstrated low levels of lymphocytes with micronuclei (LMN = 2.2 ± 0.2‰) and binucleated lymphocytes (BL = 1.9 ± 0.2‰), indicative of a stable genetic apparatus, the absence of exogenous stress on the immune system, and relatively favorable living conditions. A genetic study of the Siberian sturgeon population using four inter-microsatellite DNA markers (A – (CTC)6C, C – (AGC)6G, D – (ACC)6G, and E – (AGC)6C) revealed the presence of 117 amplicons. The informativeness of the selected ISSR markers was evaluated during the study (average values: Polymorphic Information Content (PIC) = 0.156, Effective Multiplex Ratio (EMR) = 29, Marker Index (MI) = 4.6, and Resolving Power (Rp) = 10.5). The mean number of alleles per locus (Na) was determined to be 2.00, while the effective number of alleles (Ne) was found to be 1.21. The report also presents findings on the genetic diversity of Siberian sturgeon from a local stock of the aquaculture enterprise LLC SRP "Osetr," using five microsatellite markers: Afug41, Afug51, LS19, LS34, and LS54. A total of 16 distinct multilocus genotypes (MLGs) were identified. The mean inbreeding coefficient was -0.057, indicating an excess of heterozygotes and a tendency toward outbreeding in the stock. These findings facilitate the monitoring of alterations in the genetic structure across generations and provide a basis for the avoidance of inbreeding within the farm's selective breeding program. Product Description popup.authors Bielikova Olena Yu. Borysenko Nataliia O. Hlushko Yuliia M. Mariutsa Alla E. Nahorniuk Tetiana A. Tretiak Oleksandr M. popup.nrat_date 2024-12-30 Close