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Information × Registration Number 0225U000066, (0122U001612) , R & D reports Title Phosphoribosyl isomerase gene priA as a novel tool for metabolic engineering of streptomycetes popup.stage_title Пристосування гена priA для вивчення інших видів стрептоміцетів та криптичних генних кластерів вторинного метаболізму Head Ostash Bohdan O., Доктор біологічних наук Registration Date 04-01-2025 Organization Ivan Franko National University of Lviv popup.description1 To generate a system for expression and selection of bacteria of genus Streptomyces independent of the use of antibiotics; using S. albus J1074 strain as a chassis, to demonstrate the advantages of the developed system for expression of genes for antibiotic biosynthesis and formation of antiviral nucleosides. popup.description2  Streptomycetes produce a lot of bioactive compounds that have found their use as medical, veterinary drugs, as well as chemicals in the other realms. Development of novel producers often requires their genetic engineering, and these methods are based on the use of antibiotics (usually for selection purposes). An idea of this research project is to develop a new method of Streptomyces genetic engineering that bypasses the use of antibiotics. It is proposed to build this methods around mutation in gene priA for phosphoribosyl isomerase A, which leads to inability of streptomycetes, particularly S. albus J1074, to grow in the absence of essential amino acids histidine and tryptophan. This would help simplify all methods of genetic manipulations of J1074. We constructed a set of priA mutants of J1074 and showed the ways of their use for transfer of plasmids and large fragments of the chromosome; as a tool to apply selection pressure on transcriptionally silent genes. The J1074 mutants were used as heterologous hosts to express genes for production of polyketide and other antibiotics. This led to a discovery of novel natural products. The priA-based methodology can be extended to the other streptomycete species given the high level of similarity of priA orthologs across the genus. Product Description popup.authors Hromyko Oleksandr M. Ostash Iryna S. Oleksandr S. Yushchuk popup.nrat_date 2025-01-04 Close