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Information × Registration Number 0225U000088, (0121U109268) , R & D reports Title Identification and elucidation of the role of new structural and regulatory genes in riboflavin oversynthesis in flavinogenic yeast popup.stage_title Поєднання опрацьованих підходів посилення експресії окремих структурних і регуляторних генів та класичної селекції для конструювання продуцентів рибофлавіну та оптимізація умов культивування продуцентів на різних вуглецевих субстратах. Head Sybirnyi Andrii A., д.б.н.; акад. НАНУ Registration Date 04-01-2025 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description1 The main purpose of the planned research is to study the molecular mechanisms of regulation of riboflavin (vitamin B2) oversynthesis in flavinogenic yeast and obtaining strains with increased production of riboflavin. popup.description2  Plasmids pRFE1/RIB6, pRFE1/GND1 and pRFE1/RIB6/GND1 were constructed and transformed with these plasmids into wild-type and riboflavin over-synthetic strains. Expression of different combinations of two genes, as well as co-expression of all three genes, led to an increase in riboflavin production in C. famata VKM Y-9 when grown in media of different composition. The most striking increase in riboflavin production (2-3 times) was observed with co-expression of the listed genes in the stable riboflavin-producing strain AF4. The increase in flavigenic activity of transformants with co-expression of the RFE1, RIB6 and GND1 genes in the best over-producing strain BRPI C. famata, containing over-expressed genes SEF1, RIB1, RIB7, PRS3m and ADE4m, was significantly weaker. The ability of the constructed transformants to synthesize riboflavin in industrially important media (beer wort, molasses, whey and lignocellulose hydrolysate) was tested. The highest level of riboflavin production was found in transformants with co-expressed three genes RFE1, RIB6 and GND1 in a medium with whey. The results obtained are important for creating a new concept of regulating riboflavin oversynthesis in flavinogenic yeast at the molecular level. The revealed patterns of regulation of riboflavin synthesis can be used to construct new stable overproducers by combining changes in the expression of several regulatory factors. Product Description popup.authors Bandura Yurii O. Vasylyshyn Roksolana V. Dzanaieva Liubov S. Dmytruk Kostiantyn V. Romanov Serhii O. Romanov Serhii O. Tkachenko Volodymyr I. Faiura Liubov R. Fedorenko Victor O. Fedorenko Victor O. Fedorovych Dariia V. Tsyrulnyk Andrii O. popup.nrat_date 2025-01-04 Close