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Information × Registration Number 0225U000156, (0120U101861) , R & D reports Title Pathogenetic substantiation of the practical use of the different origin stem cells in the treatment of acute cerebral ischemia (experimental study) popup.stage_title Підготовка та оформлення остаточного звіту Head Yoltukhivskyi Mykhailo V., д.мед.н. Registration Date 06-01-2025 Organization Vinnytsia National Pirogov Memorial Medical University popup.description1 The purpose of the study: to identify among the different origin stem cells the most active culture with the cerebroprotective action in the cerebral ischemia-reperfusion conditions, with subsequent determination of the neuroprotective effects dependence on cytological origin and in-depth assessment of the impact on the different brain parts with the rationale of new medicine creation. popup.description2  Report on the research work: 258p., 81 fig., 10 tab., 294 sources. The study found that allogeneic and xenogeneic transplantation of MSCs having different origin and citicoline in rats with an IR model reduce the volume of ischemic brain damage. The results obtained confirm that MSC transplantation effectively defends ischemic neurons of the penumbra zone and has certain angioprotective properties. This is due to the ability of MSCs to secrete a wide range of trophic and immunomodulatory cytokines - MSC secretom, which has a protective effect on the ischemic brain in rats through the induction of endogenous neuroprotection, neurogenesis, angiogenesis, therefore, in preclinical studies MSC transplantation was recognized as a safe and recommended recovery strategy for treatment of cerebral ischemia. It was first established that Wharton's human umbilical cord MSCs are more effective at ischemia-reperfusion injury of a brain compared to MSCs obtained from human and rat adipose tissue and are similar in the effectiveness with the cytoprotectant Citicoline. They reduced energy deficiency and metabolic acidosis in brain tissue, protected against oxidative damage to neurons, exerted a corrective effect on the exchange of nitric oxide and a modulatory effect on the activity of astro- and microglia, preserving the cytoarchitectonics of the somatosensory cortex and hippocampus (by reducing apoptosis). Also, Wharton's human umbilical cord MSCs significantly reduced neurological deficits in the subacute and recovery periods of acute cerebrovascular accident and contributed to improving the level of behavioral activity and emotionality of animals. In terms of effectiveness, rat embryonic fibroblasts were somewhat inferior to citicoline and Wharton's human umbilical cord MSCs, which indicates higher cerebroprotective activity of xenotransplantation. The demonstrated cerebroprotective properties of Wharton umbilical cord MSC transplantation are promising in cell therapy of acute Product Description popup.authors Husakova Iryna V. Yoltuhivskyi Mykhailo V. Konovalov Serhii V. Moroz Vasyl M. Stelmashchuk Alina O. popup.nrat_date 2025-01-06 Close