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Information × Registration Number 0225U000197, (0122U000580) , R & D reports Title Develop an algorithm for the use of new molecular genetic methods in the diagnostic of tuberculosis popup.stage_title Розробити алгоритм використання нових молекулярно-генетичних методів в діагностиці туберкульозу Head Zhurylo Oleksandr A., Доктор медичних наук Registration Date 07-01-2025 Organization State organization "National scientific center of phthisiatry, pulmonology and allergology named after F. G. Yanovskyi National academy of medical sciences of Ukraine" popup.description1 Increasing the efficiency of molecular genetics methods diagnosis of tuberculosis by developing algorithm for diagnosing tuberculosis and determining of M. tuberculosis drug resistance to antimycobacterial drugs popup.description2  The object of study − MBT strains from patients. The aim − to improve the efficiency of molecular genetics (MG) methods of tuberculosis diagnosis (TB) by developing TB diagnosis algorithm and determining the drug resistance (DR) of MBT to anti-tuberculosis drugs (ATDs). Methods – genetic, bacteriological, statistical, equipment – MG systems and BACTEC phenotypic system. The method was developed for the determining of the DR MBT strains to H, Q and 2-nd line injectable drugs using GeneXpert MTB/XDR cartridges, which showed high validity of the test results in comparison with LPA testing DR MBT to H and ATDs 2-nd line and fTMS, namely sensitivity, specificity, predictive value of positive and negative results (practically 100 % coincidence of results). It is proposed to use this method in the practice of TB laboratories, and to implement the method of determining the drug profile of MBT strains into the TB diagnostic algorithm. An algorithm for diagnosis and monitoring of TB treatment with DR using rapid methods has been developed. It is proposed to use rapid MG methods as initial for all cases of TB disease, as well as for the purpose of accelerated determination of of DR MBT to ATDs.. Their use makes it possible to improve the quality of research and to shorten the time period for determining DR MBT to ATDs by 2 times. The developed SOPs for carrying out whole-genome sequencing of MBT strains allow to provide genomic characterization of MBT strains, as well as to investigate genetic variants associated with drug resistance to new and repurposed drugs and an established MBT sequencing method. Application of the method makes it possible to improve the quality of laboratory studies and shorten the time period for determining of the MBT MDR before accidents by a factor of 2. Sphere of application – phthisiology, microbiology. Product Description popup.authors Barbova Anna I. Zhurylo Оleksandr A. Korzhov Vitalii I. Myronchenko Svitlana V. Trofimova Polina S. Cherednyk Yurii O. Yaremenko Mariia S. popup.nrat_date 2025-01-07 Close