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Information × Registration Number 0225U000269, (0123U100247) , R & D reports Title Study of the effect of introduced genes of proline metabolism on drought resistance and economic value traits of seed generations of winter wheat biotechnological plants popup.stage_title Перевірка успадкування ознаки осмостійкості у насіннєвих поколіннях генетично модифікованої пшениці. Молекулярно-генетичний аналіз трансформованих рослин пшениці на наявність елементів, що утворюють дволанцюговий РНК-супресор гена проліндегідрогенази пшениці і додаткової копії гена орнітин-δ-амінотрансферази люцерни. Head Mykhalska Svitlana I., Кандидат біологічних наук Registration Date 08-01-2025 Organization Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine popup.description1 Obtaining seed generations of wheat with an increased level of resistance to osmotic stresses, studying the inheritance of the trait of increased tolerance to abiotic stresses in seed generations and the influence of introduced proline metabolism genes on the physiological, biochemical and technological characteristics of the plant organism. popup.description2  The report contains 47 pages, 14 figures, 6 tables, 32 references. Object of the study. Seed generations (T1 and T4) of biotechnological plants of soft winter wheat (Triticum aestivum L.). with double-stranded RNA (dsRNA) suppressor of the proline dehydrogenase (pdh) gene and an additional copy of the alfalfa ornithine aminotransferase gene. Purpose of the work. To study the inheritance of transgenes and the preservation of the trait of increased drought and salinity resistance in seed generations T1-T4 of genetically modified wheat plants Research methods. DNA isolation, DNA PCR analysis, DNA electrophoresis, determination of proline content, analysis of yield structure indicators Results and their novelty. As a result of molecular genetic analysis of seed generations T1-T4 of genetically modified wheat, the presence of introduced elements of the exon and intron of the Arabidopsis proline dehydrogenase gene and an additional copy of the alfalfa ornithine aminotransferase gene was established, the functional activity of which determines the increase in osmoresistance due to the genetic regulation of the processes of synthesis and catabolism of proline. Successful inheritance of the acquired trait is manifested in faster seed germination and better development of genetically modified plants under conditions of osmotic stress. Among individual variants of seed generations of wheat, differences in the inheritance of the trait of increased resistance to osmotic stress were found, which may be due to the instability of the manifestation of introduced genes in generations. In a number of DNA samples, in the presence of a fragment of the first exon of the Arabidopsis proline dehydrogenase gene, the intron of this gene was not recorded, which may have caused the variability in the manifestation of the trait of increased resistance to water deficit in seed generations of biotechnological wheat plants. Product Description popup.authors Bronnikova Larysa I. Komisarenko Alla H. Kulesh Svitlana S. Mykhalskyi Leonid O. popup.nrat_date 2025-01-08 Close