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Information × Registration Number 0225U000563, (0123U100785) , R & D reports Title Study of the impact of COVID-19 on the pathogenesis and course of chronic B-cell lymphocytic leukemia in victims of the accident at the Chernobyl nuclear power plant popup.stage_title Визначення експресії легких ланцюгів імуноглобулінів залежно від стереотипії В-клітинного рецептора та впливу іонізуючого випромінення і SARS-CoV-2 Head Diahil Iryna S., Доктор медичних наукChumak Anatolii A., Доктор медичних наук Registration Date 14-01-2025 Organization State Institution "National Research Center for Radiation Medicine, Hematology and Onkology of the National Academy of Medical Sciences of Ukraine" popup.description1 To study amino acid and nucleotide structure of B-cell receptor taking into account the expression of variable regions of the heavy (IGHV) and light (IGLV) chains of immunoglobulins, their homology with anti-viral antigens, especially SARS-CoV-2, and to determine the B-cell receptor structure depend on radiation anamnesis of chronic lymphocytic leukemia patients, clinical-hematological and molecular-genetic features popup.description2  Subject: 132 chronic lymphocytic leukemia (CLL) patients, including 30 patients exposed to ionizing radiation (IR) due to the Chornobyl NPP accident (the main group), and 102 patients without IR exposure (the group of comparison). Aim: to study the expression of immunoglobulin light chain genes in groups of CLL patients depending on radiation history, B-cell receptor stereotypy, and homology with antibodies against the SARS-CoV-2 virus. Methods: clinical, molecular-genetic (polymerase chain reaction, sequences of the reaction products), statistical. Equipment: Bio-RadC1000 thermal cycler (N10450137), dispensers. It was established that the expression of IGK/LV genes in the most represented major clusters of stereotyped receptors (#1, #2, #3С3, #4, #6, #28а) was in agree with the data of other authors. For cases of clusters #5b, #9D1, #9D4, #16, #50, #59, #77, differences were found. Firstly the expression of IGK/LV genes in 55 minor clusters was revealed. The spectrum of IGK/LV genes used in the main group of CLL patients and in the group of comparison was comparable. Nucleotide sequences of 33 CLL patients had homology of IGHV genes with antiviral antibodies, but expression of IGKV/LV genes differed. Sequences with antiviral homology,compared to others, were characterized by more frequent expression of mutated IGHV genes (p = 0.00015), IGHV genes of the third family (p = 0.00006), IGHV3-21 (p = 0.0019), IGHV3-11 (p = 0.0037), IGKV2-30 (p = 0.003) and IGLV3-21 (p = 0.000065) genes, while IGHV1-69 gene expression was determined extremely rarely (p = 0.00011). Field of application: radiobiology, hematology. Publications – 3 articles, 2 abstracts, participation in two seminars. Product Description popup.authors Abramenko Iryna V. Brychenko Volodymyr V. Vatlitsov Denys V. Martina Zoia V. popup.nrat_date 2025-01-14 Close