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Registration Number

0225U000760, (0122U000375) , R & D reports

Title

Fundamental aspects of the interaction of cyclic peptide antibiotic's derivatives with nonantibiotic potentiators

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Фундаментальні аспекти взаємодії похідних циклічних пептидних антибіотиків з неантибіотичними потенціаторами

Head

Martynov Artur V., д.фарм.н.

Registration Date

18-01-2025

Organization

State Institution "I. Mechnikov Institute of Microbiology and Immunology National Academy of Medical Sciences of Ukraine"

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Determination of interaction patterns for native and modified cyclic peptide antibiotics with non-antibiotic potentiators

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 Object of the study: polymyxins, nisin, dynamic derivatives, succinyl-maleinyl polymyxins, and succinyl-maleinyl-nisin, pharmaceutical compositions of polymyxin and nisin derivatives with the addition of the potentiators amlodipine and sodium diclofenac. Purpose of the work: to study the antimicrobial activity of compositions of unmodified and modified nisin with sodium diclofenac and amlodipine against reference and clinical strains, and to investigate the stability of the developed pharmaceutical compositions. Research methods: microbiological methods, pharmaceutical technology methods, and analysis. During the conducted study: the antimicrobial properties of nisin compositions and its modified derivatives with potentiators amlodipine and diclofenac were studied concerning reference and hospital strains of microorganisms; an increase in the spectrum of antimicrobial activity of modified nisin derivatives against both reference ATCC strains and hospital strains was demonstrated; it was shown that the addition of potentiators amlodipine and diclofenac to the compositions, both native and modified nisin derivatives, increases their activity and spectrum of action. The shelf life of the extemporaneous solution of Composition No. 3 based on quasi-nisin, according to the results of stress tests, was determined to be 19 months. Further storage of the solution leads to a change in its color to light brown. The shelf life of the extemporaneous solution of Composition No. 1 (amlodipine, nisin, diclofenac), according to the results of stress tests, is 12.6 months. After this period, it no longer meets the requirements of the State Pharmacopoeia of Ukraine (SPU) regarding the level of quantitative reduction of the active pharmaceutical ingredient (API). The shelf life of the extemporaneous solution of Composition No. 2 (amlodipine, diclofenac), according to the results of stress tests, is also 12.6 months. After this period, it ceases to meet the requirements of the SPU conc

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Batrak Olena A.
Bomko Tetiana V.
Knysh Oksana V.
Martynov Artur V.
Nosalska Tetiana M.
Osolodchenko Tetiana P.
Romanova Olena A.
Sydorenko Tetiana A.

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2025-01-18