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Information × Registration Number 0225U000780, (0123U100519) , R & D reports Title Determination of optimal conditions for photodynamic inactivation of viruses popup.stage_title Визначення оптимальних умов фотодинамічної інактивації вірусів Head Kalinichenko Svitlana V., Доктор медичних наук Registration Date 18-01-2025 Organization State Institution "I. Mechnikov Institute of Microbiology and Immunology National Academy of Medical Sciences of Ukraine" popup.description1 To determine and verify optimal conditions for photodynamic inactivation of viruses on the bacteriophage model for futher development of new classes of non-toxic and non-allergenic virus-containing vaccines popup.description2 The literature on the topic of photodynamic inactivation of biological pathogenic agents (BPA) in the technologies for the manufacture and application of immunobiological preparations has been analysed. The commercial preparation Piophage® was used as a viral-containing fluid,. Photosensitizing substances were identified. The phage sensitivity of 509 clinical strains of bacteria was investigated. It was found that 47.7% of clinical isolates of E. coli, 7.6% of P. aeruginosa isolates, and 52% of S. aureus isolates were highly sensitive to Piophage®. The possibility of photo-inactivation of phage strains using solutions of riboflavin, riboflavin manadione sodium sulphate, and pyridoxine hydrochloride, or their mixture (1:1:1) at various concentrations (from 2×10^-4% to 2×10^-6%) and various modes of ultraviolet irradiation (UVO) and blue light (BL) was studied. The best inactivation parameters for the viral-containing material were obtained with the application of 0.02% and 0.01% solutions of the mixture of riboflavin, riboflavin manadione sodium sulphate, and pyridoxine hydrochloride (1:1:1) as photosensitizers under UVO for 15 minutes and 25 minutes during BL treatment. To bring the conditions of photodynamic inactivation of the viral-containing material closer to production conditions, experiments were conducted in volumes of 100 ml. Sterile disposable PVC blood bags were used for this purpose. The optimal conditions were: UVO (15 and 25 minutes) and concentrations of the mixture (1:1:1) of photosensitizers (0.02% and 0.01%). The reversion of the activity of phages did not occur over three months. Studies on the allergenicity of the obtained PDM inactivated viral-containing samples in a conjunctival test conducted on 15 animals yielded negative results. A scheme for the photo-inactivation of viral-containing material is proposed, which can be used in for the development of particulate vaccine preparations. Product Description popup.authors Kalinichenko Svitlana V. Melentieva Christina V. Ovetchyn Petro V. popup.nrat_date 2025-01-18 Close