Search academic texts

Information × Registration Number 0225U000850, (0124U002693) , R & D reports Title Microbial biologically active metabolites as a biotechnology tool to improve crop productivity and soil health recovery (MicroMet) popup.stage_title Використання біосинтетичного потенціалу мікроорганізмів для покращення живлення і розвитку рослин Head Biliavska Liudmyla O., Доктор біологічних наук Registration Date 20-01-2025 Organization Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine popup.description1 The purpose of this project is to develop the latest poly-vector microbial biotechnologies to increase the productivity of agrophytocenoses under adverse climatic changes and destructive anthropogenic influence popup.description2 The biosynthetic potential of microorganisms for improving plant nutrition and development was studied. The ability of the genus Streptomyces bacteria to synthesize a wide range of bioactive metabolites was analysed: polyene antibiotics (antifungal), phytohormones, lipids that provide growth stimulating and adaptogenic properties for plants and antagonistic properties for phytopathogens. The percentage of phospholipids, mono- and diglycerides, sterols, sterol esters and free fatty acids from the total lipid content in streptomycetes cells was determined. The total amount of auxins, cytokinins, gibberellins and abscisic acid in the biomass of producers was studied. The phytohormone-synthesizing ability of endophytic bacteria was established. The largest amount of auxins was determined for Brevibacillus sp. 5 and Paenibacillus sp. 1. Among the determined auxins, the amount of indole-3-acetic acid hydrazide was more than 90% for each of the above. Non-rhizobial soybean microsymbionts of the genera Paenibacillus, Ochrobactrum, Bacillus, Brevibacillus and Pseudomonas synthesized extracellular cytokinins (zeatin, zeatin-riboside, N6-(2-isopentenyl)adenine and N6-(2-isopentenyl)adenosine). Among the extracellular cytokinins of Paenibacillus sp. 1, Bacillus sp. 4, Brevibacillus sp. 5 and Pseudomonas sp. 6, zeatin-riboside prevailed. The activity of cytokinin biosynthesis by endophytic bacteria was lower (p<0.01, F=11.2) compared to auxins. The strain Paenibacillus sp. 3 synthesized the same amount of different cytokinin compounds on different nutrient media (p<0.1, F = 0.66). The biocontrol activity of endophytic bacteria against phytopathogenic fungi was established. The highest inhibitory activity against Alternaria alternata was shown by the supernatants of Ochrobactrum sp. 2, Bacillus sp. 4 and Pseudomonas sp. 6, and against Fusarium culmorum – Ochrobactrum sp. 2 and Brevibacillus sp. 5. The siderophore-synthesizing ability of the studied endophytes was determined. Product Description popup.authors Leonova Nataliia O. Loboda Mariia I. Tytova Liudmyla V. Shevchuk Nadiia V. popup.nrat_date 2025-01-20 Close