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Information × Registration Number 0225U001067, (0124U003592) , R & D reports Title Combining secondary bioinformatics and spatial transcriptomics to analyze the largest data set of transcriptome data of the human placenta in the course of normal pregnancy and in preeclampsia popup.stage_title Аналіз інтегрованих даних, визначення кластерів генів, біологічних процесів і генів із максимальною зміною в їх експресії Head Obolenska Mariia Yu., Доктор біологічних наук Registration Date 24-01-2025 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description1 Apply bioinformatics methods for normalization and integration of data into larger groups according to gestational periods, overcoming the problem of small samples; quantitatively characterize how the expression of genes in the placenta tissue changes between the trimesters of pregnancy in normal and preeclampsia; and in which cells, involving mRNA sequencing results in isolated placental cells, and also where differential expression takes place in 3-dimensional tissue space by in situ hybridization. popup.description2 Numerical complications of pregnancy, which are part of the Great Obstetric Syndrome, are associated with dysfunction of the placenta, this dysfunction is caused by damaged implantation of the fertilized egg and reorganization of blood vessels in the mother, which leads to changes in the blood supplied from the mother to the placenta and given to the fetus. Among the complications of the great obstetric syndrome, which is especially dangerous for the life of mothers and children, preeclampsia (PE) is widespread (accounting for 5-10% of all pregnancies in the world). The decomposition is characterized by unresolved movements of the blood pressure after the 20th stage of pregnancy. A progressive deterioration of the situation can lead to trial and death of the mother and child, which requires immediate delivery by cesarean surgery. This saves the life of the mother and child, but does not reduce their further risks. Studies of gene expression in the placenta will help to understand what processes are occurring there and to identify potential processes that may serve as markers of complexity and targets for potential rejoicing. Current molecular biology has accumulated a wealth of data from large-scale expression of genes, but due to their high variability, a small amount of research has been carried out in various laboratories around the world. The goal of the project is to develop bioinformatic methods for combining natural and openly accessible data for normalization and integration of them in a larger group along the lines of gestation. It is important to characterize how the expression of genes in the placental tissue changes between normal trimesters of gestation in order to determine the control values of the processes for further investigation of the of the complexity. This work is not only of scientific interest, but also important in view of the serious demographic situation in Ukraine. Product Description popup.authors Lykhenko Oleksandr K. Martseniuk Olha P. Obolenska Mariia Yu. popup.nrat_date 2025-01-24 Close