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Information × Registration Number 0225U001505, (0120U100936) , R & D reports Title Detection of transgenic events based on the methods of isothermal polymerase reaction and electrochemical biosensors and analysis of the effectiveness of their practical use popup.stage_title Детекція трансгенних подій на основі методів ізотермічної полімеразної реакції і електрохімічних біосенсорів та аналіз ефективності їх практичного використання Head Yemets Alla I., Доктор біологічних наук Registration Date 02-02-2025 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description1 Development of scientific principles for the application of simple and sensitive methods of detection of transgenic events in agricultural plants and food produced from them for their further use in laboratories with limited resources. popup.description2  The current state of the methodological capabilities of nucleic acid amplification at a constant temperature (isothermal amplification) was analyzed. Modern developments in the creation of 3rd generation electrochemical biosensors - biosensors with direct electron transfer were analyzed. The design and synthesis of various combinations of internal and external primers for detecting transgenic soybeans using loop-mediated amplification (LAMP) were performed. The design of electrochromic biosensors and the technology for applying thin films of non-stoichiometric tungsten oxides to their surface were studied. The conditions for conducting LAMP were selected. Laboratory biosensor samples based on electrochromic tungsten trioxide films have been developed. Databases with the sequence of internal and external primers have been created, allowing the detection of the most common transgenic lines of soybean, corn and rapeseed by the loop-mediated amplification method. A prototype of an electrochromic biosensor using thin films of non-stoichiometric tungsten oxides as electrocatalytic materials has been described. Product Description popup.authors Korhovyi Vitalii I. Levchuk Oleh M. Patsyuk Fedir N. Shysha Olena M. popup.nrat_date 2025-02-02 Close