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Registration Number

0225U001518, (0123U100962) , R & D reports

Title

Assessment of varieties and lines of soft wheat of the Forest Steppe of Ukraine by prolamin loci as donors of useful traits using molecular genetic markers and scientific and methodological support for testing genetically modified organisms

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Оцінка сортів та ліній пшениці м`якої Лісостепу України за проламіновими локусами як донорів корисних ознак з використанням молекулярно-генетичних маркерів та науково-методичне забезпечення питань випробувань генетично модифікованих організмів

Head

Blium Yaroslav B., Доктор біологічних наук

Registration Date

02-02-2025

Organization

Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine

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To evaluate varieties and lines of soft wheat of the forest-steppe of Ukraine according to prolamin loci using molecular genetic markers. Implementation of molecular genetic methods for the analysis of genetic transformation events with their simultaneous standardization, which involves the development of a platform for scientific and methodological support for the testing of genetically modified plants (organisms) (GMOs)

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 Genotypes of wheat varieties and lines of the soft zone of the Eastern and Central Forest-Steppe of Ukraine selected by the Poltava State Agrarian University (PSAU), the Bila Tserkva Research and Breeding Station of the Institute of Bioenergy Crops and Sugar Beet of the NAAS of Ukraine (BTRBS), the Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine (IPPG) were identified by the prolamin loci Gli-A1, Gli-B1, Gli-D1, Gli-A3, Glu-A1, Glu-B1, Glu-D1. A low level of heterogeneous varieties by these loci was found in the group of PSAU varieties (5%), moderate in the sample of IPPG varieties (25%) and high in the sample of BTRBS varieties (38%). The Gli-B1l allele (1BL.1RS translocation marker) is present in 17% of PDAU varieties, 35% of BTRBS varieties and 7% of IPPG varieties registered after 2010. About 18% of IPPG varieties have the wheat-rye translocation 1AL.1RS of the Amigo type. The average Glu-score is close in the analyzed groups of varieties: 8.43±0.26 in the BTRBS variety group, 8.29±0.37 in the PDAU variety group, 8.35 ±0.32 in the IPPG variety sample. A specialized GMPlantsGW database has been developed, integrated into the European network, for analyzing the presence of genetically modified components in food, feed and seeds (in particular, the collection of data on genetic modifications and methods for detecting GM plants has been updated). In an updated form, this resource is available on the Internet at the URL address: http://gmpgw.ifbg.org.ua:8080/gmodb/.

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Yemets Alla I.
Bidnyk Hanna Ya.
Buziashvili Anastasiia Yu.
Bui Denys D.
Dem'ianova Nataliia O.
Zelena Liubov B.
Karelov Anatolii V.
Karpov Pavlo A.
Kvasko Anna Yu.
Kyrylenko Viktor M.
Kozub Nataliia O.
Kurylenko Svitlana V.
Novozhylov Oleh V.
Ozheredov Serhii P.
Pirko Nadiia M.
Pastuchova Nataliia L.
Pydiura Mykola O.
Pushkarova Nadiia O.
Sakharova Vladislava H.
Sobko Tetiana O.
Sozinova Oksana I.
Spivak Svitlana I.
Filenko Oleksandr L.
Filenko Rymma O.
Khablak Serhii H.
Chuhunkova Tetiana V.
Shysha Olena M.

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2025-02-02