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Information × Registration Number 0225U001653, (0124U002158) , R & D reports Title Development of a tool for diagnosing Fusarium wilt in grain by the polymerase chain reaction (PCR) method popup.stage_title Створення науково-методичних підходів для виявлення грибів роду Fusarium за допомогою ПЛР Head Kutsan Oleksandr , Доктор ветеринарних наук Registration Date 04-02-2025 Organization Institute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine popup.description1 To develop a tool for the diagnosis of grain fusarium wilt using the PCR method, which will contribute to more effective and early diagnosis of grain fusarium wilt popup.description2  Six primer sets for the species identification of the most common species of the genus Fusarium and a pair of primers for the identification of the genus Fusarium have been developed and recommended. As a result of the conducted studies, we selected the optimal composition of the reaction mixture and the PCR mode. A comparative evaluation of the developed method using different matrices indicates a 100% coincidence of the obtained results regarding the detection of DNA of the target group of fungi of the genus Fusarium. The use of the developed approach allowed us to identify the predominant Fusarium species associated with the contamination of corn grain obtained from farms in the northern region of Ukraine. Two isolates previously assigned to the genus Fusarium were not identified at the species and genus levels. As a result of the studies, it was found that the use of species-specific primers allows us to successfully detect all target species that are the most common contaminants of corn grain. The identity of all isolates was also confirmed as the genus Fusarium, and the species distribution consisted of the predominant species: F. oxysporum (9%), F. proliferatum (13%), F. sporotrichioides (33%), F. graminearum (25%), F. verticillioides (9%) and F. culmorum (11%). Methodological recommendations "Diagnosis of grain fusarium diseases by polymerase chain reaction (PCR)" were developed and Patent for utility model No. 155838 was obtained for the method of obtaining the strain Fusarium Moniliforme Sheldon No. 3 - a producer of mycotoxins fumonisins. Product Description popup.authors popup.nrat_date 2025-02-04 Close