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Information × Registration Number 0225U001801, (0120U000223) , R & D reports Title A dynamics of the three-component system consisting of a plant, bacterium and bacteriophage at the different ecological states popup.stage_title Динаміка трьохкомпонентної системи рослина-бактерія-бактеріофаг за різних екологічних станів Head Tovkach Fedir I., Доктор біологічних наук Registration Date 07-02-2025 Organization Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine popup.description1 Creation of a general concept concerning the dynamics of complex biological objects of various degrees of complexity in the three-component system plant-bacterium-bacteriophage popup.description2 Research is aimed at creating a general concept that concerns the dynamics of interactions of biological objects of various degrees of complexity. A bioinformatics study of temperate erwiniophages 49 and 59 and lytic bacteriophage E105 was conducted. Phage 49 and 59 have the same genome size. Phage 49 and 59 show similarity in both the organization and nucleotide sequences of the genomes. The general architecture and synteny of genomes indicates that phages 49 and 59 belong to the group of lambdoid phages. Bacteriophage E105 of E. horticola belongs to lytic viruses. It lacks markers of ability to lysogeny. The genome of the lytic phage KEY of E. amylovora is 115,651 kb in size. Phage KEY belongs to the group of T5-like phages. The KEY phage together with the closely related AAS21 phage of P. agglomerans (92.2% identity) form a new genus within the Demerecviridae family. First it was shown, that the tissue transformation of willow with the "witch's broom" disease activates pseudolysogeny. Active particles of bacteriophages specific for P. agglomerans accumulate in affected plant tissues; all isolated phages belong to the B1 morphotype. Complete genome sequences of two strains of phytopathogenic bacteria P. carotovorum - Pcc ZM1 and Pcc RC5297 were obtained. The created collection of phages includes temperate phages 49 and 59, lytic phage E105 of E. horticola, T5-like phage KEY of E. amylovora, polyvalent phage FE44 and six new phages of P. agglomerans.  Product Description popup.authors Ahapov Andrii I. Bondar Tetiana V. Vasyliuk Olha M. Hnatiuk Tetiana T. Horb Tetiana Yu. Hushcha Konstantyn P. Hushcha Liubov B. Zhezheria Vladyslav A. Zlatohurska Maryna A. Kiskina Larysa Yu. Kisten Oleksandr H. Kushkina Alla I. Maksymenko Liudmyla O. Morozov Viktor L. Nasikovska Oksana P. Ochymovska Halyna M. Romaniuk Liudmyla V. Smolenska Anna P. Tkachenko Nina M. Tovkach Fedir I. Faidiuk Yuliia V. Fernati Alina V. Fernati Nataliia M. Kharkhota Maksym A. Kharchuk Maksym S. Kharchuk(Poliakova) Alina V. Cherednyk Nataliia M. Shevchenko Nadiia I. Shenderovska (Korol) Natalia A. popup.nrat_date 2025-02-07 Close