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Information × Registration Number 0225U001954, (0122U201617) , R & D reports Title Determination of the effect of ultrafast freezing on the functional viability of human mesenchymal stem cells popup.stage_title Визначити вплив методу надшвидкого заморожування на стан біохімічних процесів клітин, систем окисного відновлення, здатності до проліферації та диференціювання, тобто на функціональну життєздатність мезенхімальних стовбурових клітин людини. Head Sydorenko Myhailo V., Кандидат медичних наук Registration Date 12-02-2025 Organization State Institution Department of Biotechnical Problems of Diagnostics of the Institute of Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine popup.description1 Study of the state of mesenchymal stem cells after ultrafast freezing and storage in liquid nitrogen and development of protocols for the isolation, growth and cryopreservation of functionally viable MSCs by vitrification. popup.description2  Research report: p. - 59, fig. - 5, table - 5, references - 111. During the work, the possibility of cultivating MSCs BM on bone implants and directed osteo-differentiation of MSCs BM in the system of monolayer (2D) and spheroid (3D) growth was investigated. MESENCHYMAL STEM CELLS, MARKER EXPRESSION, MULTICELLULAR SPHEROIDS, DIFFERENTIATION Object of research: mesenchymal stem cells. The aim of the study was to compare the osteo-differentiation ability of MSCs BM in the systems of monolayer (2D) and spheroid (3D) growth. The objectives of the study were to develop protocols for cultivating MSCs in monolayer (2D) and spheroid (3D) growth systems, to compare the osteodifferentiation efficiency of MSCs in different cultivation systems and in the presence of bone substitutes. To conduct experimental directed differentiation of MSCs in monolayer (2D) and spheroid (3D) growth systems according to the osteogenic phenotype. As a result, a protocol for cultivating MSCs on bone granules was developed and tested to increase the efficiency of osteodifferentiation of cells, and the survival, proliferative potential, and ability for directed osteodifferentiation of MSCs in different cultivation conditions were compared: monolayer, spheroids, in the presence of bone substitutes. The scientific and practical significance of the work lies in the development of protocols for osteodifferentiation of BM MSCs in the presence of bone substitutes and in monolayer and spheroid growth systems, which enables more effective use of osteodifferentiated BM MSCs for bone tissue restoration in medical practice. Product Description popup.authors Bezuhlyi Serhii V. Borodai Nataliia V. Kopiak Bohnan S. Perepelytsina Anastasiia S. Siharova Nadiia V. Serheiev Oleh V. Sydorenko Мykhailo V. popup.nrat_date 2025-02-12 Close