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Information × Registration Number 0225U001955, (0121U111104) , R & D reports Title Development of a cellular test system based on multicellular spheroids generated from personalized cultures of tumor cells of patients popup.stage_title Скринінг впливу класичних хіміопрепаратів на показники утворення сфероїдів з пухлинних клітин пацієнтів. Head Sydorenko Mykhailo V., Registration Date 12-02-2025 Organization State Institution Department of Biotechnical Problems of Diagnostics of the Institute of Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine popup.description1 Development and standardization of cell test system protocols based on multicellular tumor spheroids of patients to determine the effectiveness and mechanisms of action of antitumor drugs, both those used clinically and those undergoing preclinical trials. popup.description2  Report: p. – 50, fig. – 12, table. - 1, sources of references – 61. BREAST CANCER, MESENCHYMAL STEM CELLS, marker expression, multicellular spheroids. The aim of the study was to investigate the effect of mesenchymal stromal cells during contactless co-cultivation on breast cancer cells during monolayer and spheroid growth by several parameters. Determination of morphological and phenotypic characteristics of spheroids and monolayer culture during incubation with MSCs. During the reporting period, using the developed model of tumor micronode – multicellular spheroids, a study was conducted on the effect of soluble microenvironment factors from mesenchymal stromal cells on tumor spheroids of breast cancer cells, line MCF-7. Cell survival rates in monolayer and spheroid growth models, the expression level of tumor stem cell markers and epithelial differentiation markers were compared. Object of study: tumor spheroids of the MCF-7 line, monolayer culture of the MCF-7 line, mesenchymal stromal cells during monolayer and spheroid growth. The work involved cytological, immunocytological, molecular biological methods and statistical methods of analysis. Product Description popup.authors Baresh Oleksandr M. Bezuhlyi Serhii V. Lebedieva Tamara M. Ostapenko Yurii D. Prosianyk Liudmyla B. Sydorenko Mykhailo V. Yastrebova Olena V. popup.nrat_date 2025-02-12 Close