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Information × Registration Number 0225U001999, (0123U102324) , R & D reports Title Secondary metabolites of microorganisms with antibiotic, proteolytic and glycolytic action: patterns of macromolecule organization, functional and biological activity, ways of modification and stabilization, aspects of practical use popup.stage_title Дослідити особливості продукції альфа-галактозидаз, альфа-L-рамнозидаз, еластаз, фібриногеназ, кератиназ мікроорганізмів, а також активність і стабільність ензимів за різних умов ферментації. Виділити ґрунтові стрептоміцети – продуценти антибіотиків і регуляторів їх біосинтезу. Head Varbanets Liudmyla D., д.б.н. Registration Date 13-02-2025 Organization Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine popup.description1 The aim of the work is to develop the fundamentals of obtaining microbial antibiotics, proteases, and glycosidases for the creation of technology for obtaining competitive drugs for medicine and the food industry. In order to realize this goal, the ways of production of secondary metabolites by microorganisms will be investigated, the catalytic properties of enzymes and their substrate specificity will be studied, the mechanisms of inactivation and stabilization by chemical modification will be established, the bundles of related genes for the biosynthesis of secondary metabolites in the genomes of streptomycetes will be studied. popup.description2 From the supernatant of culture liquids of Penicillium restrictum and Bacillus sp. IMV B-7883 were isolated and purified enzyme preparations with α-L-rhamnosidase and protease activity. As a result of purification, the elastase activity of Bacillus sp. IMV B-7883 was increased by 10.2 times, and fibrinogenase by 9.52 times, with a specific activity of 663.8 U/mg of protein and 180 U/mg of protein, respectively, compared to the original activity. The molecular weights of the obtained purified enzyme preparations were 23 and 20 kDa, respectively for elastase and fibrinogenase. The activity of purified α-L-rhamnosidase of P. restrictum was 27.8 U/mg of protein, and the molecular weight was 50 kDa. Optimal for the synthesis of Bacillus sp. 051 protease with elastase and fibrinogenolytic activity was a temperature of 12 °C. The maximum activity at this growing temperature is manifested on the 10-12th day of cultivation. Increasing the growing temperature (28 °C and 37 °C) contributed to a 2-fold decrease in the biosynthesis of the studied enzyme compared to growing at 12 °C. To increase the activity of enzymes, coordination compounds of germanium with various metals and gluconic acid were used, and it was shown that only the substance [М(H2O)6][Ge2(OH)2(C6H8O7)2]•nH2O (М=Ba, n=2) stimulates the activity of Eupenicillium erubescens α-L-rhamnosidase by 80%. To modify the enzymatic activity of α-L-rhamnosidase of P. restrictum, purposefully synthesized multi-ligand germanium-3d-metal complexes with citric acid, phenanthroline, and bipyridine were used for the first time. The most active compound was [Ni(bipy)3][Ge(HCit)2]•3H2O, which under experimental conditions increased the activity of P. restrictum α-L-rhamnosidase 3 times at a concentration of 0.1% and an exposure time of 24 hours. Preparations of lipopolysaccharides were obtained from Stenotrophomonas rhizophila cells, which differed in monosaccharide and fatty acid composition, but showed high pyrogenicity. Streptomy Product Description popup.authors Avdiiuk Kateryna V. Balko Olha I. Bambura Olha I. Borzova Nataliia V. Brovarska Oksana S. Bulyhina Tetiana V. Varbanets Liudmyla D. Harmasheva Inna L. Hyrenko Olha D. Holembiovsʹka Sofiya L. Hudzenko Olena V. Huliaieva Hanna B. Dankevych Liudmyla A. Dolbik Nadiia M. Zvinchuk Iryna O. Lukianchuk Vitalii V. Matseliukh Bohdan P. Nasikovska Oksana P. Pavlychenko Anna K. Polishchuk Liudmyla V. Postoliuk Tetiana H. Syrchin Serhii O. popup.nrat_date 2025-02-13 Close