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Information × Registration Number 0225U002060, (0123U101081) , R & D reports Title Synthesis of recombinant pharmaceutical proteins and increasing the content of biologically active natural compounds in plants popup.stage_title Молекулярно-біологічний аналіз отриманих трансгенних рослин, та дослідження іх антибактеріальної та антивірусної активності. Накопичення в рослинах рекомбінантних фармацевтичних білків методом транзієнтної експресії. Дослідження способів отримання біомаси та підвищення вмісту біологічно-активних речовин в рослинах родів Matricaria та Hypericum Head Kuchuk Mykola V., Доктор біологічних наук Registration Date 18-02-2025 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description1 The goal of scientific research is the creation of biotechnological plants lines that accumulate recombinant pharmaceutical proteins, in particular with antibacterial (bacteriocins) and antiviral (interferon alpha 2b and griffitsin) activity, as well as the development of biotechnologies for obtaining and increasing the biosynthesis in plant systems of natural blood-stopping, wound-healing compounds and substances with antidepressant action. popup.description2 During the project implementation, genetic vector constructs were created containing the target transgene infα2b and elements of the Potato virus X (PVX) genome to enhance transient expression and accumulate recombinant interferon in plants. Using the created expression cassette with PVX genome elements, the yield of the target protein hINF-alpha 2b was approximately 80 times higher than with a traditional expression cassette. The antibacterial activity of recombinant colicin M synthesized in transgenic kale and carrot plants was confirmed against two major enterohemorrhagic E. coli (EHEC) strains: O157:H7 and O104:H4, as well as E. coli strains resistant to beta-lactam antibiotics and carbapenems. The inheritance of the transgene was studied, and heterosis was observed in hybrids between the wild-type line and the transgenic kale line producing colicin M, resulting in a significant increase in plant biomass accumulation with antibacterial activity. Additionally, effective methods for stimulating the yield of pharmaceutical raw materials and increasing the accumulation of biologically active compounds in medicinal plants were developed. Specifically, it was demonstrated that low-dose X-ray irradiation significantly enhances the synthesis of the antibiotic chamazulene in chamomile plants; gaseous methyl jasmonate increases the content of antidepressants in aseptic Hypericum (St. John’s Wort) plants by 25–50%. The project work was conducted in accordance with high international standards using modern methods of genetic engineering and radiobiology. The results obtained are unprecedented and have no analogs worldwide. Product Description popup.authors Belokurova Valeriia B. Bulko Olha V. Halych Taras V. Zhuk Vladyslav V. Litvinov Serhii V. Lystvan Kateryna V. Loshyna Liudmyla H. Matvieieva Nadiia A. Ovcharenko Olha O. Peterson Anton A. Pchelovska Svitlana A. Rudas Volodymyr A. Sindarovska Yana R. Sakada Volodymyr I. Symonenko Yurii V. Shylina Yuliia V. Shcherbak Nataliіa L. popup.nrat_date 2025-02-18 Close