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Information × Registration Number 0225U002072, (0123U100462) , R & D reports Title Study of vital activity peculiarities of biotechnological plants after genomic modifications popup.stage_title Апробація генетичних конструкцій на модельних рослинах, вивчення експресії трансгенів та адаптивності змінених рослин до несприятливих умов зростання Head Morhun Bohdan V., Доктор біологічних наук Registration Date 18-02-2025 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description1 The purpose of scientific research is to study the possibility of genetic modification of economically useful cultivated plants with heterologous genes of various origins and to find out the peculiarities of their inheritance, phenotypic manifestation of signs, physiological and biochemical properties of bioengineered plants in comparison with the original forms. popup.description2 Genetic constructs containing a gene for phosphite oxidoreductase ptxD at the T-DNA has been tested. Correlation among the following factors was calculated – genotype, culture medium, root length, sprout length, adaptability to ex vitro conditions, and seed production in spelta. Taking into account all the indicators of in vitro culture, namely, the frequency of regeneration, rooting of regenerants, their adaptation to septic conditions and seed production, the best genotype for biotechnological research of spelta was selected that is the breeding line No. 3865 (out of 5 tested) for the use of combinations of MS/MSBA or N6/MSR (out of 8 tested) culture media for the cultivation of immature embryos. Phylogenetic analysis of spelt and bread wheat samples was performed. For the first time, a phylogenetic tree was constructed that includes not only modern zoned varieties, but also diversified varieties of wild spelt. According to the results of microsatellite analysis at loci Xgwm 219, Xgwm 261, Xgwm 383, Xgwm 469, Xgwm 508, Xgwm 626, the absence of species differences between bread wheat and spelt was shown, and, accordingly, they belong to the same species. The morphology and number of stomata on the abaxial and adaxial sides of the leaf of emmer, spelt and bread wheat of Zymoyarka variety were studied. A significant difference in the number of stomata per unit area of the adaxial surface of the leaf was found in T. aestivum of Zymoyarka variety, T. spelta var. album and T. dicoccum var. aeruginosum. The highest number of stomata per unit area of the adaxial surface of the leaf was observed in spelt, and the lowest - in wheat. On the abaxial surface of the leaf, the number of stomata per unit area was significantly higher in spelt and did not differ in wheat and spelt. Product Description popup.authors Bannykova Mariia O. Nitovska Iryna O. Pronina Olha V. popup.nrat_date 2025-02-18 Close