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Information × Registration Number 0225U002224, (0122U001442) , R & D reports Title Molecular mechanisms of activation of autophagy, apoptosis and unstructured proteins response in the gastrointestinal tract of rats with type 2 diabetes mellitus under pharmacological effects popup.stage_title Молекулярні механізми активації аутофагії, апоптозу та відгуку неструктурованих протеїнів в органах шлунково-кишкового тракту щурів із цукровим діабетом 2 типу на тлі фармакологічного впливу Head Natrus Larysa V., Registration Date 25-02-2025 Organization Bogomolets National Medical University popup.description1 Study of activation of mechanisms of intracellular homeostasis through UPR-signaling pathway, autophagy, apoptosis in cells of gastrointestinal tract of rats with experimental type 2 diabetes mellitus and against the background of pharmacological effects of short-chain FA. popup.description2  The structural and ultrastructural features of the fundic glands of the stomach of rats in an experimental model of DM2 were studied. An increase in the size of secretory granules and the appearance of phagosomes (autophagosomes) and multilamellar bodies of mucocytes were found, which can be considered as a manifestation of autophagy in the cells of the fundic glands of the stomach under the action of combination of metformin with propionate. The features of mucin production by gastric gland cells in DM2 and after therapeutic exposure were studied. A change in secretion production in producer cells was established against the background of diabetes, the combination of metformin with propionate contributed to the restoration of the balance of mucin components. The degree of functional stress in gastric glandulocytes of rats with DM2 was revealed by studying the content of the chaperone BiP/GRP78, transmembrane proteins PERK, IRE, ATF-6 in the tissue homogenate. The degree of transcription of genes of the UPR system components under the conditions of pharmacological correction with metformin in combination with propionate was studied. The concept of activation of the protective mechanism in gastric glandulocytes under of DM2 was formed based on the study of correlations between UPR sensors, comparison of the relative levels of mRNA and proteins of the UPR system in the resting state, ER stress and under the conditions of drug administration. The ultrastructural features of rat enterocytes were studied in rats with DM2 and under the conditions of pharmacological correction. The level of GRP78, PERK, IRE-1, ATF6 proteins and the degree of expression of their genes in enterocytes of the small intestine were studied in the indicated models. The level of apoptosis and proliferation markers in the small intestine of rats with DM2 after treatment with metformin and propionic acid was studied. Completely new data on the effect of propionate on the functioning of the UPR in t Product Description popup.authors popup.nrat_date 2025-02-25 Close