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Information × Registration Number 0225U002248, (0124U001897) , R & D reports Title Development of a semen thinner with antiviral properties to increase the level of biosecurity in pig farming for associated infections of Aujeski's disease and circovirus popup.stage_title Розроблення розріджувача сперми з противірусними властивостями для підвищення рівня біобезпеки у свинарстві щодо асоційованих інфекцій хвороб Ауєскі та цирковірозу Head Kolchyk Olena V., Кандидат ветеринарних наук Registration Date 26-02-2025 Organization National Scientific Center Institute of Experimental and Clinical Medicine popup.description1 The goal of the project is the development of an antiviral and antibacterial diluent for boar sperm based on nanomaterials to prevent the transfer of infection (viral and bacterial) to the genital tract of a female with boar sperm through its preliminary sanitation before use in the artificial insemination process. popup.description2  In the project in 2024, the effectiveness of the drug composite (amiksin + metisazone) in the composition of the boar sperm diluent against Aujeszky's disease virus (HA) on sensitive transplanted cultures and its antibacterial properties were determined. Cytological, virological, molecular genetic and bacteriological methods were used in the research. Studies were conducted to determine the non-toxic concentration of the drug on sperm and its bactericidal effect. The (composite) was prepared and its dilution in the saline solution 1:10; 1:100; 1:1000; 1:10000; 1:100000; The recipe is according to the standard recipe ("amiksin + metisazone"). During the research, the viability of sperm batches with motility of at least 70% was assessed and the effect of the composite on sperm in various dilutions was determined. According to the results of the studies, a non-toxic concentration of the composite was determined, which was 1:100. Effective bactericidal concentration of the composite was determined, which was 1:1000. It was established that the greatest decrease in the titer of infectious activity of the HA virus (by 2.54 TCD50/cm3) was observed after its reproduction in cell culture with its simultaneous introduction into the cell monolayer in the maximum permissible concentration (3.0 μg/cm3 of nutrient medium). The maximum effect of the drug on the hemagglutinating activity of the HA virus (a decrease of more than 3.0 log2) was observed after cultivation of the pathogen in a transfected cell culture in its maximum concentration. It was determined that the composite exhibited antiviral activity against HA (inhibition of CPD, effect on infectious and hemagglutinating activity) Product Description popup.authors Buzun Andrii I. Kuprienko Iraida V. Pavlenko Bohdan M. Sazonenko Svitlana M. Yurko Polina S. popup.nrat_date 2025-02-26 Close