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Information × Registration Number 0225U002293, (0120U100849) , R & D reports Title Targeted genome changes and pleiotropic effects in genetically transformed plant systems popup.stage_title Дослідження експресії перенесених генів, плейотропних ефектів та фенотипічних змін у отриманих біотехнологічних рослин Head Kuchuk Mykola V., Доктор біологічних наук Registration Date 02-03-2025 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description1 The aim of scientific research is to purposefully change the genome of economically valuable plants of various species by the method of genetic transformation, to study phenotype changes, gene inheritance features and pleiotropic effects resulting from the expression of heterologous genes. popup.description2  During the reporting period, an analysis of biotechnological plants and root lines of various species was conducted to confirm the expression of transferred genes, transgene inheritance, and potential pleiotropic effects associated with transgenesis. The effect of temperature factors on the growth and bioactive compound content in hairy roots of Bidens pilosa L. was investigated. In transgenic Bidens pilosa roots, an increase in flavonoid content, enhanced antioxidant activity, and differences in response to temperature stress were observed. These differences are considered potential pleiotropic effects resulting from the expression of rol genes from Agrobacterium rhizogenes in root cultures. At the same time, transgenic tomato plants of the "Shedevr 1" variety carrying the human interferon α-2b gene, as well as lettuce and kale plants expressing the antibacterial protein colicin M, showed no morphological differences from the control group, indicating the absence of pleiotropic effects related to the expression of the recombinant pharmaceutical protein in these plants. Similarly, no pleiotropic effects or phenotypic changes were observed in biotechnological rapeseed plants obtained through genetic transformation with the epsps gene, which confers glyphosate herbicide resistance. The obtained results meet high international standards and contribute valuable insights into the stability and safety of transgenic plants for agricultural and pharmaceutical applications. Product Description popup.authors Bulko Olha V. Vasylenko Maksym Yu. Hnatiuk Iryna S. Duplii Volodymyr P. Lystvan Kateryna V. Luchakivska Yuliia S. Loshyna Liudmyla H. Maistrov Petro D. Nadiia А. Matvieieva Nifantova Svitlana M. Ovcharenko Olha O. Potrokhov Andrii O. Ratushniak Yakiv I. Rudas Volodymyr А. Sindarovska Yana R. Symonenko Yurii V. Shakhovskyi Anatoli M. Shcherbаk Nаtаliia L. Yaroshko Olha M. popup.nrat_date 2025-03-02 Close