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Information × Registration Number 0225U002701, (0122U002115) , R & D reports Title Mechanisms of stress adaptation and creation of resistance plant lines by genetic engineering methods popup.stage_title Молекулярно-біологічний аналіз отриманих трансгенних рослин; визначення наявності перенесених генів в рослинному геномі етодом ПЛР; підтвердження експерсії трансгенів на рівні транскрипції методом ЗТ-ПЛР Head Kuchuk Mykola V., д.б.н. Registration Date 30-03-2025 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description1 The aim of the project is to create plants with better adaptive characteristics through genetic transformation of vectors containing genes, the expression of which can increase the resistance of plants to stress. popup.description2  During the reporting period, genome editing (targeted mutagenesis) of Desiree potato genes was carried out using the CRISPR/Cas9 genome editing system to obtain biotechnological plants with enhanced pathogen resistance. Genetically modified maize plants were obtained, containing the Cry-toxin gene sequence in their genome, which provides resistance to insect pests. A study of C-repeat binding factor (CBF) transcription factor genes in duckweed was conducted, demonstrating that the increase in CBF mRNA levels under high-osmotic stress conditions is species-dependent. The highest expression was observed in the CBF2 gene of Lemna punctata, a duckweed species known for its high stress tolerance. Additionally, experiments with duckweed plants showed that NH₄⁺ in the nutrient medium (compared to NO₃⁻) alleviates Mn-stress symptoms in Spirodela polyrhiza by reducing the expression of transporter genes located in the plasmalemma, which are responsible for ion uptake into the cell. In experiments with transgenic N. benthamiana plants expressing the ZRNase II gene, a significant slowdown in the systemic spread of the virus and suppression of viral replication in transgenic plants were demonstrated. The project was conducted in accordance with high international standards, utilizing modern methods such as sequencing, bioinformatics analysis of genetic sequences, in vitro culture, genetic transformation techniques, and genome editing. During the reporting period, genome editing (targeted mutagenesis) of Desiree potato genes was carried out using the CRISPR/Cas9 genome editing system to obtain biotechnological plants with enhanced pathogen resistance. Genetically modified maize plants were obtained, containing the Cry-toxin gene sequence in their genome, which provides resistance to insect pests. A study of C-repeat binding factor (CBF) transcription factor genes in duckweed was conducted, demonstrating that the increase in CBF mRNA levels under high-osmotic stress Product Description popup.authors Kishchenko Olena М. Kyrpa Tetiana M. Lystvan Kateryna V. Ovcharenko Olha O. Potrokhov Andrii O. Rudas Volodymyr А. Symonenko Yurii V. popup.nrat_date 2025-03-30 Close