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Information × Registration Number 0225U002727, (0122U002115) , R & D reports Title Mechanisms of stress adaptation and creation of resistance plant lines by genetic engineering methods popup.stage_title Дослідження стійкості отриманих трансгенних рослин до відповідних стресових чинників Head Kuchuk Mykola V., д.б.н. Registration Date 01-04-2025 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description1 The aim of the project is to create plants with better adaptive characteristics through genetic transformation of vectors containing genes, the expression of which can increase the resistance of plants to stress. popup.description2  The study involved the development of biotechnological plants with enhanced resistance to a range of stress factors such as pathogens, toxins, and herbicides. In particular, CRISPR/Cas9 gene editing was applied to the potato cultivar Désirée to obtain plants with increased resistance to pathogens. Gene knockouts were performed for CML30, CML38, and WAK12, which play a role in the plant immune response. The results showed that gene modifications affected the morphological characteristics of the plants. Additionally, the study examined the toxicity of manganese (Mn) for Spirodela polyrhiza duckweed, a plant with potential for phytoremediation. It was found that increased Mn concentrations activate biochemical pathways influencing plant metabolism. Herbicide-resistant lines of winter rapeseed were also developed, incorporating genes conferring resistance to glyphosate and phosphinothricin. Furthermore, the role of the ZRNase II gene in enhancing plant resistance to phytoviruses was investigated. It was found that the expression of ZRNase II increases resistance of transgenic potato and petunia plants to phytoviruses. Product Description popup.authors Kishchenko Olena М. Kyrpa Tetiana M. Lystvan Kateryna V. Ovcharenko Olha O. Peterson Anton A. Potrokhov Andrii O. Rudas Volodymyr А. Symonenko Yurii V. popup.nrat_date 2025-04-01 Close