Search academic texts

Registration Number

0225U002806, (0123U101319) , R & D reports

Title

Ionic, molecular and membrane mechanisms of reduction in the fertilization potential of spermatozoa and its correction

popup.stage_title

Йонні, молекулярні та мембранні механізми зниження фертилізаційного потенціалу сперматозоїдів чоловіків та його корекція

Head

Fafula Roman V., Доктор біологічних наук

Registration Date

06-04-2025

Organization

Danylo Halytsky Lviv National Medical University

popup.description1

Find out the biophysical and biochemical mechanisms of disturbances of the fertilizing ability of spermatozoa in various forms of infertility and to propose new criteria for the diagnosis of infertility based on the determination of the activity of a number of enzymatic systems of spermatozoa and the concentration of secondary messengers

popup.description2

A decrease in the Ca2+,Mg2+-ATPase activity was found in men with idiopathic infertility and infertile men with concomitant autoimmune joint pathology. A direct significant correlation between the activity of the thapsigargin-resistant component of Ca2+,Mg2+-ATPase and sperm motility was found (r = 0.55; p < 0.05). The diagnostic significance of ATPase activity parameters in the diagnosis of male infertility was determined. It was shown that TBARS content was 4.0 times (p<0.001) higher in the seminal plasma of infertile men with autoimmune pathology of the joints compared to fertile men, while in patients with idiopathic infertility its level was within the normal range. The activation of lipid peroxidation processes was accompanied by a statistically significant decrease in the enzymes activity of the glutathione link of antioxidant defense in infertile men with idiopathic infertility and concomitant rheumatoid arthritis. More pronounced changes in lipid peroxidation and antioxidant enzyme activity were observed in seminal plasma compared to blood plasma. The cNOS activity in seminal plasma and blood lymphocytes was reduced in both study groups compared to the control group. The decrease in cNOS activity was accompanied by a sharp increase in iNOS activity. The arginase activity in seminal plasma was reduced in patients with idiopathic infertility compared to fertile men in the control group. It was established that idiopathic infertility was associated with a decrease in the activity of non-oxidative, arginase metabolism of L-arginine and the dominance of the NO synthase pathway, which leads to hyperproduction of nitric oxide. There was an increase in the arginase/NOS ratio in lymphocytes in infertile men with rheumatoid arthritis, which is largely due to a sharp activation of arginase activity. The ratios of arginase/NOS and iNOS/cNOS activities can be used as indicators of oxidative and nitrative stress in men with various forms of infertility.

Product Description

popup.authors

Vorobets Dmytro Zinoviyovych
Воробець Зіновій Дмитрович
Melnyk Oksana V.
Nakonechnyi Yosyf A.
Onufrovych Olena K.
Fedorovych Zoriana Ya.

popup.nrat_date

2025-04-06