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Information × Registration Number 0225U002982, (0124U002438) , R & D reports Title Application of phytobacterial communities for bioremediation of ecosystems popup.stage_title Дослідження ефективності фітобактеріального методу очищення забруднених ґрунтів за використання багаторічних рослин для акумуляції металів та ріст-стимулювальних мікроорганізмів роду Pseudomonas для мобілізації металів у ризосфері ґрунту Head Havryliuk Olesia A., Доктор філософії Registration Date 28-04-2025 Organization Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine popup.description1 Development of a phytobacterial method for the remediation of contaminated soils and investigation of the influence of microorganisms on the efficiency of metal accumulation by plants. The work is dedicated to the search for methods to increase the efficiency of phytoremediation of soils, in particular by using soil microorganisms of the genus Pseudomonas. The uniqueness of the work lies in the integrated approach where the metabolic potential of both plants (ability to accumulate metals) and bacteria (ability to mobilise metals) will be used to remediate metal-contaminated ecosystems. popup.description2 In this work, a complex called "Phyto-Bac" was developed, comprising both effective phytobacteria (Pseudomonas lactis UKR1, Pseudomonas panacis UKR2, Pseudomonas veronii UKR3 and UKR4) and field-grown phytoremediator grasses for the recovery of degraded land. The purpose of this development was to reduce the toxic load on agroecosystems and to create favourable conditions for the restoration of soil biodiversity. Experiments involving the Pseudomonas bacterial consortium demonstrated that the presence of the inoculant enhanced the plants' ability to accumulate copper. At a contamination level of 100 mg/kg, copper extraction efficiency increased from 13% to 27.8%; at 500 mg/kg, from 8.5% to 15.5%; and at 1000 mg/kg, from 0.8% to 2.1%. However, at 1000 mg/kg of copper, plant mortality occurred in both inoculated and uninoculated variants. The use of the Pseudomonas inoculant nearly doubled the phytoremediation efficiency under low and medium levels of contamination, and nearly tripled it under high contamination. Additionally, changes in the population size of Pseudomonas bacteria in the soil were observed depending on the conditions of inoculation and copper contamination. The presence of the bacterial inoculant supported bacterial growth even under metal stress conditions. Analysis of soil metabolic parameters, including pH and redox potential (Eh), revealed that copper contamination led to soil acidification, particularly in the absence of microbial inoculation. Meanwhile, the presence of the Pseudomonas inoculant stabilised soil pH, reduced acidity, and improved conditions for plant growth. Product Description popup.authors Bida Iryna O. popup.nrat_date 2025-04-28 Close