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Information × Registration Number 0225U002983, (0124U002438) , R & D reports Title Application of phytobacterial communities for bioremediation of ecosystems popup.stage_title Загальна характеристика ріст-стимулювальних ізолятів Pseudomonas, перспективних для використання біологічного методу очищення та відновлення забруднених ґрунтів Head Havryliuk Olesia A., Доктор філософії Registration Date 28-04-2025 Organization Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine popup.description1 Development of a phytobacterial method for the remediation of contaminated soils and investigation of the influence of microorganisms on the efficiency of metal accumulation by plants. The work is dedicated to the search for methods to increase the efficiency of phytoremediation of soils, in particular by using soil microorganisms of the genus Pseudomonas. The uniqueness of the work lies in the integrated approach where the metabolic potential of both plants (ability to accumulate metals) and bacteria (ability to mobilise metals) will be used to remediate metal-contaminated ecosystems. popup.description2  In this study, the "Phyto-Bac" complex was developed, combining effective phytobacteria (Pseudomonas lactis UKR1, Pseudomonas panacis UKR2, Pseudomonas veronii UKR3 and UKR4) with field phytoremediator grasses for the restoration of degraded land. The goal of this development was to reduce the toxic load on agroecosystems and to create favourable conditions for the recovery of soil biodiversity. Experiments with the Pseudomonas bacterial consortium showed that the presence of the inoculant improved the plants’ ability to accumulate copper. At a concentration of 100 mg/kg, copper extraction efficiency increased from 13% to 27.8%; at 500 mg/kg, from 8.5% to 15.5%; and at 1000 mg/kg, from 0.8% to 2.1%. However, at 1000 mg/kg of copper, plants died in both experimental variants. The application of the Pseudomonas inoculant nearly doubled the efficiency of phytoremediation at low and medium levels of contamination and nearly tripled it at high contamination levels. Changes in the number of Pseudomonas bacteria in the soil were also observed under different inoculation and copper contamination conditions. The presence of the inoculant promoted bacterial growth even under metal stress conditions. The study of soil metabolic parameters, particularly pH and redox potential (Eh), showed that copper contamination led to soil acidification, especially in the absence of microbial inoculation. At the same time, the presence of the Pseudomonas inoculant helped stabilise soil pH, reducing acidity and improving conditions for plant growth. Similarly, the redox potential of the soil decreased with inoculation, indicating the bacteria’s ability to regulate redox processes and reduce the toxic effects of copper. Thus, the bacterial strains and plant species included in the "Phyto-Bac" complex demonstrated high effectiveness in copper-contaminated soils and are promising for practical use in agroecological and bioremediation programmes. Product Description popup.authors Bida Iryna O. popup.nrat_date 2025-04-28 Close