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Information × Registration Number 0225U003035, (0123U103248) , R & D reports Title Bacterial diseases of nut crops and flower plant varieties introduced in Ukraine under different climatic conditions popup.stage_title Моніторинг, відділення в чисту культуру, ідентифікація за патогенними, морфолого-культуральними, фізіолого-біохімічними властивостями та жирно-кислотним складом клітинних ліпідів збудників бактеріальних хвороб горіху волоського і окремих квіткових культур (еустома, керемек, гвоздика) Head Hnatiuk Tetiana T., Кандидат біологічних наук Registration Date 07-05-2025 Organization Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine popup.description1 The purpose of the research is to assess the species composition of the population of bacterial pathogens of walnut and some new varieties of flower crops (eustoma, keremek, cloves) introduced in Ukraine under the influence of various natural and climatic conditions, intense military operations; to study the complex of pathogen properties for their rapid identification and diagnosis. popup.description2 In accordance with the objectives of the work plan for 2023, the following results were obtained: 1.17 strains of bacteria were isolated as a result of monitoring for bacterial diseases of walnut trees in 12 regions of Ukraine, as well as areas close to the line of hostilities (Dnipropetrovska oblast, Prosiana village, Kherson). The preliminary identification revealed that Xanthomonas arboricola pv. juglandis was detected in 70% of the studied regions, Pseudomonas syringae pv. syringae in 29% and Agrobacterium tumefaciens in 1%. 2.The range of potential host plants for newly isolated pathogens was determined by artificial infection. For isolates from walnut, carrot pieces and Kalanchoe leaves were infected, which determined the relationship of a number of strains to the genus Agrobacterium; and on tobacco leaves, there was an RNase characteristic of bacteria of the genus Pseudomonas; the manifestation of symptoms during artificial infection of walnut trees and horse beans - related some strains to representatives of the genus Xanthomonas. For three pathogenic strains from eustoma, infection of eustoma and kermek notched was carried out. Two strains showed high virulence to both plants, which indirectly confirms their preliminary identification as Burkholderia caryophylli. The third strain showed medium aggressiveness to eustoma and low aggressiveness to notched kermek. 3.According to the results of determining the fatty acid (f.a.) composition of cellular lipids, it was found that: 1) f.a. spectra of bacterial strains isolated from walnut are similar to the typical and 11 collection strains of A. tumefaciens, 3 collection strains of X. arboricola pv. juglandis and the type strain of P. syringae pv. syringae; 2) the f.a. spectrum confirms the affinity of two strains to representatives of the genus Burkholderia and the affinity of the third strain to B. gladioli or Pseudomonas fluorescens is being determined in comparison with the literature data. Product Description popup.authors Hnatiuk Tetiana T. Zarudniak Mykola I. popup.nrat_date 2025-05-07 Close