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Information × Registration Number 0225U003198, (0122U001831) , R & D reports Title Spectra and biological activity of secondary metabolites by the interaction of microorganisms in the biofilm popup.stage_title Спектри і біологічна активність вторинних метаболітів за взаємодії мікроорганізмів у біоплівці Head Filipova Tetiana O., д.б.н. Registration Date 31-05-2025 Organization Odesa I.I. Mechnikov National University popup.description1 To determine the spectra and biological activity of secondary metabolites of microorganisms by their interaction in marine biofilms. Performe modeling of natural and artificial consortia and establish prospects and feasibility of using co-cultivation in biotechnological processes. popup.description2  The biological activity of secondary metabolites from mono- and co-cultures of bacteria isolated from biofilms on the surface of mussels from the Black Sea was investigated, along with their synthesis of proteolytic enzymes. It was established that the secondary metabolites of co-cultures exhibit a higher level of antimicrobial, anti-biofilm, and antitumor activity compared to the metabolites of monocultures. It was also shown that co-cultures contain significantly more siderophores than the expected sum of those from the monocultures. Additionally, it was found that the surfactant activity of the secondary metabolites from co-cultures in oil displacement and dilution tests is higher than that of the metabolites derived from monocultures. The most significant scientific results were the obtained evidence for the activation of silent genes responsible for the synthesis of secondary metabolites by marine bacteria. The most effective approaches to this were: co-cultivation of different bacterial species, the use of seawater in the composition of the growth media, aeration, and the application of biofilm cultures. Thin-layer chromatography revealed the presence of lipopeptides and glycolipids among the secondary metabolites from both mono- and co-cultures. In two samples of secondary metabolites, the appearance of pigments, likely flavonoids, was observed, which were not present in monocultures. Mass spectrometric analysis of the mixtures of secondary metabolites showed that new compounds, absent in monocultures, appear in co-cultures. In one case, in the co-culture of Pseudomonas sp. with Bacillus sp., the disappearance of one of the Bacillus metabolites was recorded. The results indicate the prospects for further study of marine microbiota as producers of beneficial secondary metabolites. Product Description popup.authors Vodzinskyi Serhii V. Halkin Borys M. Halkin Mykola B. Sashchuk Olena V. Semenets Anastasia S. Filipova Tetiana O. Finohenova Mariia O. Shtenikov Mykola D. popup.nrat_date 2025-05-31 Close