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Information × Registration Number 0225U003487, (0123U101139) , R & D reports Title Study of the regenerative potential of cell therapy in orthopedics and traumatology popup.stage_title Експериментальний етап Head Holovakha Maksym L., д.мед.н. Registration Date 02-07-2025 Organization Zaporizhzhya State Medical University popup.description1 Тo study and justify the use of autologous cell cultures for the treatment and recovery of trauma and orthopedic patients. popup.description2  It was found that in patients, mesenchymal stem cells with the immunohistochemical profile CD105+, CD73+, CD34+, CD31-, CD45- are present in the stromal vascular fraction (SVF) of both the infrapatellar Hoff body and subcutaneous adipose tissue. The number of these cells in the SVF of subcutaneous adipose tissue is statistically significantly higher by 4.38 times than in the SVF of the Hoff body (p <0.05).The relative number of CD31+, CD105+, CD34+ cells in the SVF of Hoff's fat body and the SVF of subcutaneous fat tissue did not have statistically significant differences (p > 0.05), while the relative number of CD73+, CD45- cells was statistically significantly higher in the SVF of Hoff's fat body than in the SVF of subcutaneous fat tissue (1.7 and 1.4 times, respectively). In experimental rats, the popliteal and intrapulmonary arteries with perivascular adipose tissue (PAFT) responded to acute hypoxia with vasoconstriction – an increase in the amplitude of contraction in the first and second phases; in the case of PAFT, they responded with a decrease in the maximum amplitude of contraction in phase 1 and an increase in the amplitude in phase 2. In the samples of the studied arteries of rats with type 2 diabetes, in response to hypoxia, a significant increase in the content of nitrotyrosine and a violation of the expression of NOS isoforms were observed - an increase in the expression of iNOS mRNA and a decrease in the expression of eNOS mRNA. Removal of PVAT from the vessels of rats with type 2 diabetes led to more pronounced changes in the nitric oxide system. In the future, it is possible to medically correct the effect of perivascular adipose tissue on vascular vasoconstriction, which will improve regeneration processes. Local SVF therapy creates conditions for tissue restoration and proliferation by activating the body's own regenerative capabilities, including cell growth and differentiation, which is confirmed by clinical observations. Product Description popup.authors Avramenko Yuliia M. Bezverkhnii Andrii A. Bukhtiiarova Nina V. Lisunov Mykhailo S. Maslennikov Serhii O. Chornyi Vladyslav M. popup.nrat_date 2025-07-02 Close