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Information × Registration Number 0225U003649, (0125U002233) , R & D reports Title Microbiological substantiation for the development of means of combating antibiotic-resistant pathogens of infectious complications associated with the provision of medical care popup.stage_title Мікробіологічне обґрунтування розробки засобів боротьби з антибіотикорезистентними збудниками інфекційних ускладнень, пов’язаних з наданням медичної допомоги Head Nazarchuk Oleksandr A., Доктор медичних наук Registration Date 25-07-2025 Organization Nazarchuk Oleksandr A. popup.description1 increasing the effectiveness of combating antibiotic-resistant pathogens of infectious complications associated with the provision of medical care by studying their biological properties and microbiological substantiation for the development and use of alternative antimicrobial agents. popup.description2  The research is devoted to solving the scientific and practical problem of developing effective alternative approaches to improve the fight against antibiotic-resistant pathogens of healthcare-associated infections (HAIs). Based on the microbiological examination of biomaterial from 478 surgical patients with burns and post-traumatic wounds (2023-2025), 634 strains of microorganisms were isolated. The prevalence of gram-negative bacteria (76.7%). Gram-positive bacteria (21.6%) were presented mainly by Staphylococcus aureus (69-83%). Multidrug resistance was detected in predominant bacteria: A. baumannii (100% to penicillins, cephalosporins); P. aeruginosa (95.7-100% to penicillins, cephalosporins, fluoroquinolones, aminoglycosides, 87-97.8% to carbapenems); K. pneumoniae (100% to penicillins, cephalosporins, fluoroquinolones, 36.7-58.3% to carbapenems). The correlation between phenotypic and genotypic resistance among priority pathogens was shown: A. baumannii (r=0.90, p<0.0001), K. pneumoniae (r=0.60, p<0.03), P. aeruginosa (r=-0.17, p<0.53). The high antimicrobial properties of modern QAC antiseptics were established. The significant advantages antimicrobial efficacy of decamethoxine in combination with the local anesthetic drug bupivacaine was proved, in particular, their synergistic antimicrobial effect and the ability to reduce biofilm formation by 3.7-21.3% (p≤0.001). The obtained data allowed us to substantiate the feasibility of their use as alternative agents in the fight against antibiotic-resistant strains of priority pathogens of UTIs. Biomaterials based on calcium alginate with decamethoxine (0.2-0.8%) and bupivacaine were developed and showed high antimicrobial activity (p≤0.001). The practical significance of the study is the scientific substantiation of the local use of antisepticі and the investigation of biomaterials based on them, which is a promising alternative in the context of antibiotic resistance. Product Description popup.authors Nazarchuk Oleksandr A. popup.nrat_date 2025-07-25 Close