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Information × Registration Number 0225U003735, (0121U107836) , R & D reports Title The substantiation of the principles of biochemical and DNA markers selection for the genotypes resistance assessment of Vitis vinifera L. grapes to fungal diseases agents popup.stage_title Добір та встановлення ефективності біохімічних та мікросателітних ДНК-маркерів стійкості до збудника оїдіуму Uncinula necator Head Muliukina Nina А., Доктор сільськогосподарських наук Registration Date 11-08-2025 Organization National Research Center "VE Tairov Institute of Viticulture and Enology" popup.description1 The aim of the project is to identify and select effective biochemical and DNA markers for resistance screening of grapevine selection material (Vitis vinifera L.) to fungal pathogens. The project identifies grape powdery mildew as the main target of disease control treatments by pesticides in plant protection schemes and grapevine esca, a fungal trunk disease of perennial wood, one of the most effective means of control of which is the sanitary certification of grapevine planting material. Different degrees of the causative agents' research lead to the need of DNA identification use for the esca agents because in contrast to European vine-growing countries for Ukraine this question remains open. The use of molecular markers of resistance to esca will focus on biochemical markers, primarily grape stilbenes. To assess the resistance of grape genotypes to powdery mildew, microsatellite DNA markers of resistance genes will be used and the most resistant grape genotypes will be evaluated (according to the field resistance assessment) selected by NSC “Tairov Research Institute of Viticulture and Wine-Making». The second step in identifying molecular markers of resistance to powdery mildew will be grape polyphenolic compounds screening, the concentration of which may be a sign that can be quickly assessed in hybrid populations. The final result of the project will be the database of molecular markers of resistance to grapevine fungal diseases and guidelines for the use of this database in breeding programs for resistance to fungal diseases of grapes creation. popup.description2  The research report includes 25 pages, 3 tables, 4 figures, 48 references. Object of study: grape varieties Subject of study: resistance to powdery mildew Material: technical and table grape varieties Methods: analysis of origin, field assessment of the level of resistance to powdery mildew, determination of peroxidase and chitinase activity Analysis of the origin of the group of varieties of the V. E. Tairov Scientific and Technological Research Center and determination of field resistance levels demonstrated that a number of variety samples are descendants of species carrying genes for powdery mildew resistance (the share of relatively powdery mildew-resistant genotypes in the repository is 28%). Agrobiological assessment of the resistance of new selection varieties to powdery mildew on a 9-point evaluation scale has indicators from 6.8 to 7.5 points. The pedigrees of these varieties, according to the International Catalogue of Grape Varieties “Vitis”, mostly include V. rupestris and V. amurensis. It has been shown that peroxidase activity can serve as a marker of resistance to powdery mildew in grapes, and according to this indicator, the following table and technical varieties resistant to powdery mildew are distinguished: ʻPerseusʼ, ʻFontaneʼ, ʻZagreiʼ, ʻAromatnyʼ, ʻIdylia muscatnaʼ, ʻOdessky Zhemchugʼ and ʻYaryloʼ. For some varieties of the sample, the use of the chitinase enzyme as a protein marker of powdery mildew resistance was also shown to be promising. The sources of resistance of grape varieties to powdery mildew were analyzed and, based on literature data and origin analysis, microsatellite DNA markers were selected to assess the resistance of grape genotypes to powdery mildew Keywords: technical and table grape varieties, powdery mildew, peroxidase, chitinase, R-genes. Product Description popup.authors Baranets Liudmyla O. Karastan Olha M. Leshchenko Alla O. Mezerniuk Taras M. Mulyukina Nina A Papina Olena S. Sliusarenko Oleksandr M. popup.nrat_date 2025-08-11 Close