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Registration Number

0225U003744, (0121U107836) , R & D reports

Title

The substantiation of the principles of biochemical and DNA markers selection for the genotypes resistance assessment of Vitis vinifera L. grapes to fungal diseases agents

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Оцінка та підбір груп резистентних до грибних хвороб та чутливих генотипів Vitis vinifera L.

Head

Muliukina Nina А., Доктор сільськогосподарських наук

Registration Date

11-08-2025

Organization

National Research Center "VE Tairov Institute of Viticulture and Enology"

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The aim of the project is to identify and select effective biochemical and DNA markers for resistance screening of grapevine selection material (Vitis vinifera L.) to fungal pathogens. The project identifies grape powdery mildew as the main target of disease control treatments by pesticides in plant protection schemes and grapevine esca, a fungal trunk disease of perennial wood, one of the most effective means of control of which is the sanitary certification of grapevine planting material. Different degrees of the causative agents' research lead to the need of DNA identification use for the esca agents because in contrast to European vine-growing countries for Ukraine this question remains open. The use of molecular markers of resistance to esca will focus on biochemical markers, primarily grape stilbenes. To assess the resistance of grape genotypes to powdery mildew, microsatellite DNA markers of resistance genes will be used and the most resistant grape genotypes will be evaluated (according to the field resistance assessment) selected by NSC “Tairov Research Institute of Viticulture and Wine-Making». The second step in identifying molecular markers of resistance to powdery mildew will be grape polyphenolic compounds screening, the concentration of which may be a sign that can be quickly assessed in hybrid populations. The final result of the project will be the database of molecular markers of resistance to grapevine fungal diseases and guidelines for the use of this database in breeding programs for resistance to fungal diseases of grapes creation.

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 The research report is presented on 20 pages, contains 5 tables, 4 figures, 11 literature sources. Object of research – grape varieties The aim of the research was to identify and select effective biochemical and DNA markers for accelerated screening for resistance of grape breeding material Vitis vinifera L. to pathogens of fungal diseases. Research methods: field and laboratory, determination of pathogen resistance of varieties, genetic analysis of the origin and inheritance of resistance genes, microsatellite DNA markers. Research results It was found that a number of varieties selected by the NSC "IViV named after V.E. Tairov", which are descendants of the species V. rupestris, V. amurensis, V.labrusca, V. berlandieri and others, can serve as donors of genes for resistance to mildew and oidium, in particular, Rpv3, Rpv10, Rpv12. The high level of field resistance of new selection varieties to pathogens of fungal etiology was confirmed on a 9-point scale (indicators from 6.8 to 7.5 points (including table varieties of 5-6 generations of crosses Perseus, Odyssey, Riddle, Comet, Langeron and others). Based on the results obtained, a joint research program was developed with the Institute of Viticulture (Tekirdag), which includes hybridization and molecular genetic evaluation of parental forms and hybrid populations with three markers (Rpv3, Rpv10, Rpv12) associated with the manifestation of the mildew resistance trait in grapes; Keywords: ampelographic collections, molecular markers, mildew, oidium.

Product Description

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Baranets Liudmyla О.
Karastan Olha M.
Muliukina Nina A.
Papina Olena S.

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2025-08-11