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Information × Registration Number 0225U004030, (0118U005177) , R & D reports Title Development of the smart-sensing device based on the novel nanocomposite films for the detection of creatinine and ammonium ions. Part 2. Development of an amperometric printed smart sensor for the simultaneous determination of creatinine and ammonium ions. popup.stage_title Метрологічні дослідження портативних креатинінових аналізаторів та підготовка комплекту науково-технічної документації на прилади. Head Korpan Yaroslav I., Кандидат біологічних наук Registration Date 21-10-2025 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description1 popup.description2 Laboratory prototypes of chemosensors for the determination of ammonium ions based on printed graphite electrodes, Cu2O nanoparticles and polyaniline were created and their main analytical characteristics were studied (sensitivity (at the level of 2000 A•M-1•m-2), selectivity, stability (more than 100 measurements without loss of sensitivity)). Methods for immobilization of the recombinant (His)6-tagged form of creatine deiminase on the surface of graphite amperometric electrodes, pre-modified with the ammonium-sensitive nanocomposite PANi/Cu2O, were developed and tested. Laboratory prototypes of enzyme sensors for the determination of creatinine were created and their main analytical characteristics were studied (sensitivity, selectivity, stability, reproducibility, etc.). An experimental sample of a printed creatinine electrode was tested in clinical serum samples of patients and conditionally healthy individuals and the main characteristics were compared with other methods for determining the target molecule. A metrological evaluation of printed sensor analytical systems for the simultaneous determination of ammonium and creatinine ions was carried out. Procedures for determining the molar concentrations of the above-mentioned analytes in serum samples were developed. Product Description popup.authors Korpan Yaroslav I. Biloivan Olha A. popup.nrat_date 2025-11-03 Close