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Information × Registration Number 0225U004046, (0124U004567) , R & D reports Title Novel Portable Non-Invasive Real-Time Monitoring System for Biomarkers Detection in Preterm Infants popup.stage_title Розробка проекту та технічного завдання на портативну систему моніторингу. Розробка та характеристика біосенсорів. Head Dzyadevych Serhii V., Доктор біологічних наук Registration Date 29-10-2025 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description1 Development of novel portable monitoring systems that uses polymeric materials, optical and/or electrochemical biosensors for non-invasive continuous, accurate and sensitive assessment of specific biomarker in preterm infants popup.description2 The design and technical requirements for a portable monitoring system have been developed, as well as the necessary characteristics of various biosensors required for introduction into such a system. For this purpose, promising approaches have been identified for the analysis of very low concentrations of analytes in dialysate. The development of a number of electrochemical monobiosensors for the determination of lactate, bilirubin, glucose and pyruvate has been initiated. In order to further combine all monobiosensors into a single system for the simultaneous determination of all 4 analytes, a single version of electrochemical converters has been selected. Based on the results of testing and analysis of various designs of electrochemical converters, amperometric sensors based on disk platinum electrodes were selected, which are easily modified by polymer and enzyme membranes, and the technology of combining them into complex multisystems is known and not very complicated. Accordingly, all monobiosensors have a similar design, but differ in the enzyme in the composition of the bioselective element and the method of immobilization of this enzyme. The monobiosensor for glucose determination is based on glucose oxidase, and the lactate-sensitive sensor contains lactate oxidase. The biosensor for pyruvate determination is developed based on pyruvate oxidase, and the bilirubin sensor - using bilirubin oxidase. Laboratory prototypes of amperometric biosensors based on glucose oxidase, lactate oxidase and pyruvate oxidase for the determination of glucose, lactate and pyruvate, respectively, have been developed and characterized. The first version of the biosensor based on bilirubin oxidase for the determination of bilirubin has been developed. Product Description popup.authors Liudmyla V. Shkotova Oleksandr O. Soldatkin Daryna O. Mruha popup.nrat_date 2025-11-03 Close