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Registration Number

0225U004180, (0123U102893) , R & D reports

Title

To study the effect of metals and non-metals of nanoparticles and composite materials on the biological activity and expiration dateof of various taxonomic groups microorganisms

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Дослідження репродуктивних властивостей вірусів, бактерій, грибів за дії наночастинок металів, неметалів та композитних матеріалів для полдальшої розробки ветеринарних препаратів на їх основі

Head

Bolotin Vitalii I., Кандидат ветеринарних наук

Registration Date

21-11-2025

Organization

State Scientific-Control Institute of Biotechnology and strains of microorganisms

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To study the reproductive properties of viruses, bacteria, fungi under the action of metals, non-metals of nanoparticles and composite materials for further development of veterinary drugs based on them. A database on the reproductive activity of viruses, bacteria and fungi under the action of metals, non-metals of nanoparticles and composite materials, which can be used for the development of veterinary drugs. Based on the results of the research, a final report will be drawn up. The main research results will be published in scientific publications indexed in the Web of Science Core Collection or Scopus scientometric databases.

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The study of the effect of nanoparticles and their composites on cell cultures of the СНЕВ and ВНК-21 lines was conducted. The maximum permissible concentrations of nanoparticles and nanomaterials for СНЕВ and ВНК-21 cell cultures were determined. The ability of Fe II nanoparticles to increase the metabolic activity and proliferation of ВНК-21 cells at a concentration of 25-12.5 was shown, which when adding Fe II nanoparticles was 45.9% higher than in the control. Work was carried out on the corresponding cell cultures of model viruses to determine the effect of individual nanoparticles on the reproductive activity of viruses. In particular, the teshovirus strain Perechinsky 642, the transmissible gastroenteritis virus of pigs (TGS) strain 70, the bovine coronavirus strain S379 Reims, the Aujeszky's disease virus strain Bartha K61 were refreshed, worked up and titrated for research. Electron microscopy of the cultivated strains of tehovirus and coronavirus (TCS) was performed. The antiviral effect of nanoparticles, in particular Se+I, S+I, Se+S+I, La, Ce, Ni, Ti, Fe II and ShS, on Aujeszky's disease virus was established. It was found that Fe II nanoparticles after incubation for an hour at a temperature of 37 oC in a 1:1 dilution with a suspension of native virus completely stop the replication of Aujeszky's disease virus in SNEV cell culture, and Ti, Ce, Se+S+I nanoparticles and ShS nanoparticles under the same conditions reduce the titer of Aujeszky's disease virus by 3.27, 3, 2.16 and 2.05 lg TCD50/ml, respectively, compared with the control. The minimum concentrations of nanoparticles that affect the replication of Aujeszky's disease virus in SNEV cell culture were determined. Thus, the minimum concentration of Se+I nanoparticles that affected the replication of Aujeszky's disease virus and prevented the development of cytopathic effect of the virus in the culture of SNEV cells was 12.5+25 μg/ml, Se+S+I ‒ 10+15+25 μg/ml, Ce ‒ 40 μg/ml, Ni ‒ 35 μg/ml.

Product Description

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Aleksandr D. Nebeschuk
Oleksandr М. Kornieikov
Yuliia V. Shevel
Yana A. Rodina
Nazarii R. Kordupel
Olena V. Sychova

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2025-11-21