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Registration Number

0225U004193, (0124U001557) , R & D reports

Title

Study of descending regulation of sensory neuron activity in different types of dorsal horn surface laminae and lamina X, in normal conditions and in the development of chronic pain syndromes.

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Встановлення ролі низхідних проекцій та гомо- та гетеросегментального пресинаптичного інгібуванні нейронів DH спинного мозку в нормі.

Head

Romanenko Serhii V., Кандидат біологічних наук

Registration Date

25-11-2025

Organization

Bogomolets Institute of Physiology of the National Academy of Sciences of Ukraine

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A poor understanding of the mechanisms of chronic pain hinders effective treatment! The spinoparabrachial pathway (SPp) and rostral ventromedial area of the brainstem (RVM) are the key spinal cord structures and higher sensory processing areas, that are involved in the processing of nociceptive information and presumably in the formation of the pathological syndrome of chronic pain, yet their functions and connections remain poorly understood. This study aims to find out how pain information is processed and modulated in the SPp, RVM and other brain regions, as well as how different subgroups of RVM neurons are involved in the process of chronic pain of different etiologies. The project also aims to investigate changes in excitatory and inhibitory synaptic transmission in heterogeneous neurons of the dorsal horn (DH) superficial laminae with the involvement of ON-neurons and OFF-neurons in the RVM, and similar interaction with limina X neurons. The ultimate goal of the proposed study is to develop new feasible, specific and highly effective strategies for the treatment of pain syndromes of various genesis and to create new pharmacological approaches and medications for the treatment of pain.

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This study examined the impact of descending projections from the rostroventromedial trunk (RVM) on spinal cord X neurons responsible for transmitting pain signals. While these projections are recognized as critical regulators of nociceptive information, the underlying mechanisms by which they exert their effects have not been fully elucidated. An ex-vivo study on spinal cord preparations showed that stimulation of the RVM via the dorsolateral funiculus (DLF) inhibits Aδ- and C-afferent inputs and reduces network activity in lamina X. Under conventional dorsal root ganglion (DR) stimulation, lamina X neurons showed a significant increase in spontaneous synaptic activity. However, moderate DLF stimulation partially suppressed this activity, while intense stimulation reduced it to baseline levels. Lamina X, which contributes to the transmission of nociceptive information, was thoroughly examined. Research has demonstrated that its function is predominantly mediated by AMPA receptors, with NMDA receptors serving a more auxiliary role. Activation of TRPV1 receptors on primary afferents markedly enhances synaptic activity within lamina X neurons, suggesting direct afferent projections to this region.

Product Description

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Tetiana A. Pivneva
Serhii V. Romanenko
Vasyl P. Yatsenko
Olena V. Bagatska
Olha I. Kostiuk
Valeria A. Mitruzaeva
Oleh V. Halaidych
Dmytro Y. Duzhyi
Julia O. Dobropolska
Kostiantyn V. Koroid
Yana O. Naumenko

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2025-11-25