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Registration Number

0225U004223, (0120U105434) , R & D reports

Title

Tumor-associated genes for the diagnosis of malignant tumors and the development of gene therapy approaches in model systems

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Біоінформативний аналіз даних для пошуку потенційних генів-супресорів росту пухлин, пухлино-асоційованих генів та генетичних порушень генів, пов'язаних з резистентістю до ліків. Створення колекції зразків пухлин сечового міхура, передміхурової залози та інших типів епітеліальних пухлин. Аналіз генетичних та епігенетичних змін, а також експресії відібраних кластерів генів.

Head

Kashuba Volodymyr I., Доктор біологічних наук

Registration Date

26-11-2025

Organization

Institute of Molecular Biology and Genetics of NAS of Ukraine

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Identification and functional characterization of new potential genes-suppressors of tumor growth and tumor-associated genes, the change in the expression of which accompanies the emergence and progression of human tumors for the possible diagnosis of tumors of epithelial origin. Development and creation of a high-tech vector genetic engineering system based on recombinant baculoviruses, study of vector cells for targeted delivery of target genes to block the progression of malignant tumors in model systems and study the impact of suppressor genes on the development of malignant tumors.

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Research report: 41 p., 2 tables, 5 figures, 48 ​​sources. The object of the study is prostate cancer cell lines; prostate tumor samples and urine from patients with the corresponding diagnosis. The purpose of the work is to analyze genetic and epigenetic changes in potential tumor growth suppressor genes and tumor-associated genes. The search is aimed at identifying genes for the diagnosis of malignant neoplasms and the development of gene therapy approaches in model systems. A comprehensive approach was used: bioinformatic data analysis, experimental research methods and statistical processing of results. A collection of samples of bladder, prostate and other types of tumors was created. A bioinformatic data analysis was performed to search for potential tumor growth suppressor genes, tumor-associated genes and genetic disorders of genes associated with drug resistance. The methylation of the promoters of potential PCA growth suppressor genes - KRT18, VIM, CDH1, RASSF1A, PTEN, NKX3.1 and GDF15 - was studied in tumor tissue and urine of PCA patients. It was shown that the set of methylated PTEN, CDH1, NKX3.1, RASSF1A and GDF15 based on the obtained calculations may be suitable for discriminating PCA patients from individuals without cancer. New combinations of biomarkers based on methylation of tumor suppressor genes for the diagnosis of PCA with high sensitivity and specificity are proposed. The results of the studies indicate the possibility of using methylation of tumor suppressor genes as non-invasive markers of PCA.

Product Description

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Olena A. Zaharuk
Larysa I. Chashchyna
Oksana S. Mankovska
Inna V. Rosokhatska
Liudmyla D. Khrebtiievska
Tamara M. Haidaienko
Yurii V. Vahin
Hanna V. Gerashchenko
Vasyl V. Gordiiuk
Iryna M. Vahina
Oksana V. Maksymchuk
Mykola O. Chashchyn
Maiia T. Bobrovska
Vitalii V. Kashuba

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2025-11-26