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Registration Number

0225U004224, (0120U105434) , R & D reports

Title

Tumor-associated genes for the diagnosis of malignant tumors and the development of gene therapy approaches in model systems

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Вивчення експресії, метилювання промоторних ділянок для білок-експресуючих генів та генів некодувальних РНК. Характеристика пухлино-супресорної активності відібраних генів in vitro.

Head

Kashuba Volodymyr I., Доктор біологічних наук

Registration Date

26-11-2025

Organization

Institute of Molecular Biology and Genetics of NAS of Ukraine

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Identification and functional characterization of new potential genes-suppressors of tumor growth and tumor-associated genes, the change in the expression of which accompanies the emergence and progression of human tumors for the possible diagnosis of tumors of epithelial origin. Development and creation of a high-tech vector genetic engineering system based on recombinant baculoviruses, study of vector cells for targeted delivery of target genes to block the progression of malignant tumors in model systems and study the impact of suppressor genes on the development of malignant tumors.

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The object of the study is tissue and urine samples from prostate cancer (PC) and bladder cancer (BC); tissue samples from breast cancer (BC); baculovirus expression system. The aim of the work is to analyze genetic and epigenetic changes in tissues and urine from prostate cancer (PC) and bladder cancer (BC); in tissues from breast cancer (BC); to characterize the potential tumor suppressor properties of TAGLN in the baculovirus expression system in vitro. The search is aimed at identifying genes for the diagnosis of malignant neoplasms and the development of gene therapy approaches in model systems. 1. The expression of the RASSF1A genes, long non-coding RNAs ANRASSF1, CCAT1 and GAS5 in tumors and conditionally normal tissues in prostate cancer and bladder cancer was studied. 2. The molecular genetic characteristics of the structure of the BRCA1 gene were determined and hypermethylation of its promoter was detected in breast cancer. 3. The level of expression of the ZFP36 gene and its methylation in breast cancer were studied. 4. The tumor suppressor functions of the TAGLN gene were confirmed and various mechanisms of the influence of this gene on cell death were identified. The results of the studies indicate the possibility of using genetic and epigenetic changes in the studied genes (changes in expression at the RNA and protein levels; methylation status of promoters of protein-coding genes and long non-coding RNA genes) as non-invasive markers for the diagnosis of malignant neoplasms and the development of gene therapy approaches in model systems.

Product Description

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Olena A. Zaharuk
Larysa I. Chashchyna
Oksana S. Mankovska
Inna V. Rosokhatska
Liudmyla D. Khrebtiievska
Yurii V. Vahin
Hanna V. Gerashchenko
Vasyl V. Gordiiuk
Iryna M. Vahina
Oksana V. Maksymchuk
Maiia T. Bobrovska
Vitalii V. Kashuba

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2025-11-26