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Registration Number

0225U004226, (0120U105434) , R & D reports

Title

Tumor-associated genes for the diagnosis of malignant tumors and the development of gene therapy approaches in model systems

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Продовження забору біологічного матеріалу та поповнення колекції зразків. Продовження аналізу генетичних, епігенетичних змін та експресії у відібраних кластерах генів. Дослідження бакуловірусних векторів з метою вдосконалення доставки цільових генів в пухлинні клітини. Вивчення властивостей різних клітин для відбору векторних клітин, що експресують досліджувані гени. Клонування в бакуловірусній системі низки генів-супресорів та пухлино-асоційованих генів.

Head

Kashuba Volodymyr I., Доктор біологічних наук

Registration Date

26-11-2025

Organization

Institute of Molecular Biology and Genetics of NAS of Ukraine

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Identification and functional characterization of new potential genes-suppressors of tumor growth and tumor-associated genes, the change in the expression of which accompanies the emergence and progression of human tumors for the possible diagnosis of tumors of epithelial origin. Development and creation of a high-tech vector genetic engineering system based on recombinant baculoviruses, study of vector cells for targeted delivery of target genes to block the progression of malignant tumors in model systems and study the impact of suppressor genes on the development of malignant tumors.

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The object of the study is the isoforms of the proteins PDL1 (programmed cell death-ligand 1 (CD274)) and PDCD1 (programmed cell death 1(CD279)) and genes of the cytochrome P450 family in prostate tumor samples. The aim of the work is to study the relationship between the relative expression levels (BE) of different isoforms of the proteins PDL1 and PDCD1, the clinicopathological characteristics of prostate tumor samples and other tumor- and immune-associated genes; to study the relative expression of genes of the cytochrome P450 family (CYPs) in prostate cancer. The search is aimed at identifying genes for the diagnosis of malignant neoplasms and the development of gene therapy approaches in model systems. A comprehensive approach was used: bioinformatic data analysis, experimental research methods and statistical processing of results. 1. An information search for cytochrome P450 isoenzymes was carried out, since CYP-dependent metabolites regulate the cell cycle, proliferative, invasive and migratory activity of cancer cells. 2. The relative expression levels of different isoforms of the PDL1 and PDCD1 genes and 14 genes of the cytochrome P450 family in prostate tumors were determined; significant correlations were found between the relative expression levels of the studied genes and the clinicopathological characteristics of tumor samples and the expression of dozens of tumor- and immune-associated genes. 3. It was shown that the relative expression levels of short isoforms of PDCD1 and PDL1 have positive correlations with age. 4. The properties of various human and animal cells were studied for the selection of vector cells expressing the studied genes, in particular TAGLN, IFNB, IL21. The obtained data indicate that before prescribing immunotherapy methods, it is desirable to assess the levels of relative expression of long isoforms of the PDCD1 and PDL1 genes in order to analyze the expected sensitivity of tumors to such treatment.

Product Description

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Olena A. Zaharuk
Larysa I. Chashchyna
Ganna Gerashchenko
Oksana S. Mankovska
Inna V. Rosokhatska
Liudmyla D. Khrebtiievska
Tamara M. Haidaienko
Yurii V. Vahin
Vasyl V. Gordiiuk
Iryna M. Vahina
Oksana V. Maksymchuk
Mykola O. Chashchyn
Maiia T. Bobrovska
Vitalii V. Kashuba

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2025-11-26