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Registration Number

0225U004330, (0120U105434) , R & D reports

Title

Tumor-associated genes for the diagnosis of malignant tumors and the development of gene therapy approaches in model systems

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Характеристика активності відібраних потенційних генів-супресорів роста пухлин та пухлино-стромальних взаємодій. Вивчення протипухлинного ефекту пухлино-асоційованих генів у модельних системах.

Head

Kashuba Volodymyr I., Доктор біологічних наук

Registration Date

01-12-2025

Organization

Institute of Molecular Biology and Genetics of NAS of Ukraine

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Identification and functional characterization of new potential genes-suppressors of tumor growth and tumor-associated genes, the change in the expression of which accompanies the emergence and progression of human tumors for the possible diagnosis of tumors of epithelial origin. Development and creation of a high-tech vector genetic engineering system based on recombinant baculoviruses, study of vector cells for targeted delivery of target genes to block the progression of malignant tumors in model systems and study the impact of suppressor genes on the development of malignant tumors.

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The object of the study is tissues and urine of patients with PCM and PC. The aim of the work is to study the expression of a number of long non-coding RNAs in tumor tissues and urine of patients with bladder and prostate cancer and in exosomes of various origins. The search is aimed at identifying genes for diagnosing malignant tumors and developing gene therapy approaches in model systems. A decrease in the relative abundance of RASSF1A gene transcripts and ANRASSF1 antisense dnRNA was found in urine samples of patients with PCM. A strong positive correlation of ANRASSF1 with RASSF1A expression in tumor tissues in PCM was demonstrated. Lower expression levels for ANRASSF1 were found in urine samples of patients with bladder cancer compared to healthy individuals. Changes in the BE of SNHG1 dnRNA in tissues of the corresponding tumors and the relative level of this transcript in urine samples of patients with PCM were found. It was determined that the relative expression of PTEN and SNHG1 dnRNA was higher in tumor tissues of bladder cancer. A negative correlation of SNHG1 dnRNA in urine and treatment was found. A statistically significant negative correlation was found between GAS5 expression in normal tissues and Gleason score. Increased expression of PANDAR in bladder cancer tissues was shown compared to paired normal tissues. A negative correlation was found in patients with prostate cancer between urinary PANDAR levels and PSA levels. An exosome segregation method was developed to study changes in bladder and prostate cancer. The relative level of dnRNA was determined in exosomes isolated from conditioned medium of cell lines, urine and plasma of prostate cancer patients. A number of candidate genes for the regulation of heparin sulfate biosynthesis were identified. The results of the studies indicate that long non-coding RNAs, in particular ANRASSF1 and SNHG1, have diagnostic and prognostic potential in bladder cancer.

Product Description

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Roman V. Gulkovskyi
Larysa I. Chashchyna
Liliia V. Mevs
Hanna V. Herashchenko
Oksana S. Mankovska
Inna V. Rosokhatska
Liudmyla D. Khrebtiievska
Yurii V. Vahin
Vasyl V. Hordiiuk
Iryna M. Vahina
Oksana V. Maksymchuk
Maiia T. Bobrovska
Vitalii V. Kashuba

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2025-12-01