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Information × Registration Number 0225U004358, (0123U102740) , R & D reports Title Silvicultural and molecular genetic principles of increasing the biotic resilience of Scots pine stands in the context of adaptation to climate change and anthropogenic pressure popup.stage_title Лісівничі та молекулярно-генетичні засади підвищення біотичної стійкості деревостанів сосни звичайної в контексті адаптації до змін клімату та антропогенного навантаження Head Kovalova Valentyna A., Кандидат біологічних наук Registration Date 02-12-2025 Organization Ukrainian national forestry university popup.description1 To develop scientific and methodological bases of biotechnological applications aimed at growing biologically stable pine forests in Ukraine under new ecological conditions by introducing close-to-nature forestry, to determine the role of defensin genes in the resistance of pine to complex Ips acuminatus-Ophiostoma for further application in breeding programs, as well as to investigate the adaptogenic properties of bacteria for increasing the resistance of pine seedlings to stress popup.description2 Within this project, a comprehensive study of Scots pine stands was conducted for the first time across the landscape-geographical regions of the Lviv oblast, integrating forest typology, stand assessment, physiological, molecular-genetic, phytopathological, entomological, and biotechnological analyses under conditions of climate change and anthropogenic pressure. Using Landsat satellite imagery, detailed thematic maps were created to illustrate the dynamics of pine stands across age groups, providing an important tool for monitoring and planning forest management activities. For the first time, the species composition of phytophagous insects in pine young stands was examined, and the distribution in Ukraine of the Ophiostoma clavatum complex of ophiostomatoid fungi and the phytopathogen Fusarium verticillioides—both associated with the pine shoot beetle—was documented. Transcriptomic profiling of Scots pine defensin genes and bioinformatic analysis of their promoters revealed, for the first time, the high stress-responsive potential of this multigene family, identifying these genes as promising candidates for selecting stress-tolerant genotypes. Promising biotechnological approaches for plant protection and growth stimulation were developed: the endophytic bacteria Pseudomonas putida P57 and P. lurida P88 were isolated and characterized. Approaches to close-to-nature forestry were elaborated, emphasizing the transition from monocultures to mixed, uneven-aged stands. The project results, supplemented by a FAIR-compliant dataset, provide a scientific foundation for developing effective measures to strengthen the biotic resilience of pine ecosystems and for shaping modern strategies of sustainable forest management amid climate change and post-war recovery. Product Description popup.authors Yuliia I. Shalovylo Yusypovych Yurii M. Hryhorii T. Krynytskyi Krynytska Olha H. Mykola M. Korol Kit Oleh Yu. Volodymyr K. Mahuran Vasyl V. Lavnyi Volodymyr O. Kramarets popup.nrat_date 2025-12-02 Close