Search academic texts

Registration Number

0225U004391, (0123U103772) , R & D reports

Title

Biophysical characteristics of circulating metastatic cells as potential targets of antimetastatic therapy

popup.stage_title

Дослідження впливу активації ентозу (дефіцит глюкози) та інгібування ентозу (агент Y-27632) на виживаність, біофізичні характеристики та гетерогенність популяції клітин LLC та LLC/R9 за їх деадгезивного росту in vitro.

Head

Dovbeshko Halyna I., д.ф.-м.н.

Registration Date

03-12-2025

Organization

Institute of Physics of National Academy of Sciences of Ukraine

popup.description1

The aim of the work is to determine biophysical and biochemical characteristics, the changes of which are correlated with the viability of circulating tumor cells and to experimentally substantiate the possibility of using these characteristics as potential therapeutic targets for antimetastatic therapy.

popup.description2

Subject of study: Lewis lung carcinoma cell cultures with high (LLC) and low (LLC/R9) metastatic potential. The purpose of the study: Determination of biophysical characteristics and experimental substantiation of the possibility of using these characteristics as potential therapeutic targets for antimetastatic therapy Research methods and equipment: biochemical methods, cytofluorimetric method using labeled monoclonal antibodies, IR and Raman spectroscopy, confocal and CARS microscopy. Theoretical and practical results: Studies have shown differences in the mechanical properties of the membranes of LLC and LLC/R9 cells depending on their cultivation conditions and under the influence of energy metabolism inhibitors. It has also been shown that deadhesive cells have a more densely packed membrane. The pressure required for complete cell penetration into the capillary is 20% higher than for adhesive cells. A greater spread of the curves of the dependence of the applied pressure on the aspiration radius for adhesive cells was also noted, which can be explained by their higher proliferative heterogeneity. Thanks to the use of an innovative method of drying at the critical point of cells for electron microscopy, it was possible to record fine morphological structures in the form of filopodia and lamellopodia, as well as visualize protein-lipid complexes (lipid droplets) using the CARS microscopy method and correlate morphological and structural rearrangements with spectral features.

Product Description

popup.authors

Romanenko Serhei V.
Solianyk Halyna I.
Halyna I. Dovbeshko
Olenchuk Maryna V.
Barabash Yurii M.
Serhii O. Karakhim
Olexandra O. Lykhova
Olena P. Gnayuk
Kolesnyk Denys L.
Stepanov Yurii V.

popup.nrat_date

2025-12-03