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Information × Registration Number 0225U004466, (0121U107917) , R & D reports Title Development of technology for induction of gametophyte reduced parthenogenesis by apozygotic method of seed reproduction and obtaining homozygous heterofertile lines for hybrid selection of sugar beets popup.stage_title Розроблення технології індукції гаметофітного редукованого партеногенезу при апозиготичному способі репродукції насіння і отримання гомозиготних роздільноплідних ліній для гібридної селекції буряків цукрових Head Roik Mykola V., д.с.-г.н. Registration Date 08-12-2025 Organization Institute of Bioenergy Crops and Sugar Beet of National Academy of Agrarian Sciences of Ukraine popup.description1 To develop methods of induction of haploids and double haploids and to isolate homozygous single-fruited lines of sugar beets with cytoplasmic male sterility and apozygotic method of seed reproduction to create hybrids, without the use of sterility fixers. popup.description2 Haploid seedlings were isolated by morphometric indicators in apomictic progeny of sugar beet, the percentage of which varied from 9.2% to 60.4%, depending on the genetic origin of the material. Haploid lines propagated in vitro were characterized by the presence on the histograms of AP "Partec", both haploid and diploid cells, in the G2 period of the cell cycle, which differed from haploids obtained from immature seed embryos. In terms of the level of genome ploidy, they stabilized to the diploid level within 2-3 passages using selection by ploidy, based on cytophotometric indicators. Interspecific hybrids based on new sterile cytoplasms of Beta maritima and Beta patula used as haploinducers were isolated by the method of gynogenesis using grafted tester genes of hypocotyl color R+r- of one-year and two-year development cycles B+b-. A homozygous line of ChS II Beta vulgaris YADSS A1. p2 XXzzmmr-r-b-b- with a high ability to induce haploids was isolated for use as a tester in the selection of marker genotypes of haploinducers. Product Description popup.authors Mykola V. Roik Nataliia S. Bekh Liliia H. Hritsyshina Nataliia S. Kovalchuk Volodymyr M. Hymentyk Olesia А. Zinchenko Yavnyuk Olena M Iryna I. Boyko Nailia R. Humerova Oksana V. Hryshchenko Nadiia P. HONChARUK Svitlana V. KhIMICh popup.nrat_date 2025-12-08 Close